Woo K B, Funkhouser W K, Sullivan C, Alabaster O
Cell Tissue Kinet. 1980 Nov;13(6):591-604. doi: 10.1111/j.1365-2184.1980.tb00498.x.
The in vitro proliferation kinetics of a cell line derived from a patient with American Burkitt's lymphoma were investigated at three different growth phases: lag (day 1), exponential (day 3) and plateau (day 5). The growth curve, labeling and mitotic indices, percentage labeled mitosis (PLM) curves and DNA content distributions were determined. The data obtained have been analysed by the previously developed discrete-time kinetic (DTK) model by which a time course of DNA distributions during a 10-day growth period was characterized in terms of other cell kinetic parameters. The mean cell cycle times, initially estimated from PLM curves on days 1, 3 and 5, were further analysed by the DTK model of DNA distributions and subsequently the mean cell cycle times with respect to DNA distributions during the entire growth period were determined. The doubling times were 39.6, 31.2 and 67.2 hr, respectively, at days 1, 3 and 5. The mean cell cycle time increased from 23.0 to 37.7 hr from day 3 to day 5 mainly due to an elongation of the G1 and G2 phases. A slight increase in the cell loss rate from 0.0077 to 0.0081 fraction/hr was accompanied by a decrease in the cell production rate from 0.0299 to 0.0184 fraction/hr. This calculated cell loss rate correlated significantly with the number of dead cells determined by trypan blue exclusion. Analysis of the number of dead cells in relation to the cell cycle stage revealed that a majority of cell death occurred in G1 (r + 0.908; P < 0.0001). There was a good correlation between the in vitro proliferation kinetics at plateau phase of this Burkitt's lymphoma derived cell line and the in vivo proliferation kinetics of African Burkitt's lymphoma (Iversen et al., 1974), suggesting the potential utility of information obtained by in vitro kinetic studies.
对源自一名美国伯基特淋巴瘤患者的细胞系在三个不同生长阶段（停滞期（第1天）、指数生长期（第3天）和平台期（第5天））的体外增殖动力学进行了研究。测定了生长曲线、标记指数和有丝分裂指数、标记有丝分裂百分比（PLM）曲线以及DNA含量分布。通过先前开发的离散时间动力学（DTK）模型对获得的数据进行了分析，利用该模型根据其他细胞动力学参数对10天生长期间的DNA分布时间进程进行了表征。最初根据第1天、第3天和第5天的PLM曲线估算的平均细胞周期时间，通过DNA分布的DTK模型进一步分析，随后确定了整个生长期间相对于DNA分布的平均细胞周期时间。在第1天、第3天和第5天，倍增时间分别为39.6、31.2和67.2小时。平均细胞周期时间从第3天到第5天从23.0小时增加到37.7小时，主要是由于G1期和G2期延长。细胞丢失率从0.0077分数/小时略有增加到0.0081分数/小时，同时细胞产生率从0.0299分数/小时下降到0.0184分数/小时。该计算出的细胞丢失率与通过台盼蓝排斥法测定的死细胞数量显著相关。对与细胞周期阶段相关的死细胞数量分析表明，大多数细胞死亡发生在G1期（r = +0.908；P < 0.0001）。该伯基特淋巴瘤衍生细胞系在平台期的体外增殖动力学与非洲伯基特淋巴瘤的体内增殖动力学之间存在良好相关性（Iversen等人，1974年），表明体外动力学研究获得的信息具有潜在用途。
