Johny K V, Dasgupta M K, Singh B, Dossetor J B
Clin Exp Immunol. 1980 Jun;40(3):459-68.
A new method for the detection of circulating immune complexes using radiolabelled conglutinin as a marker for complement-bound complexes precipitated in low concentrations (3.5%) of polyethylene glycol is described. The optimal laboratory conditions for the test have been defined and certain limitations studied. The technique is simple with good reproducibility and high degree of specificity. In a double-blind study of eighty-seven samples of sera from normals, patients with glomerulonephritis, post-renal transplantation, rheumatic diseases, breast tumours, multiple sclerosis and infections the method was compared and correlated to the Clq-binding assay and the Raji cell test. The radioconglutinin-binding assay was most discriminative for cases of glomerulonephritis, renel transplantation, breast carcinoma and multiple sclerosis and least for rheumatoid arthritis.
本文描述了一种检测循环免疫复合物的新方法，该方法使用放射性标记的胶固素作为补体结合复合物的标记物，这些复合物在低浓度（3.5%）的聚乙二醇中沉淀。已确定了该检测的最佳实验室条件，并研究了某些局限性。该技术操作简单，具有良好的重现性和高度特异性。在一项对87份来自正常人、肾小球肾炎患者、肾移植后患者、风湿性疾病患者、乳腺肿瘤患者、多发性硬化症患者和感染患者的血清样本的双盲研究中，将该方法与Clq结合试验和Raji细胞试验进行了比较和相关性分析。放射性胶固素结合试验对肾小球肾炎、肾移植、乳腺癌和多发性硬化症病例的鉴别能力最强，而对类风湿性关节炎的鉴别能力最弱。
