Eckhardt R, Hopf U, Hütteroth T H, Meyer zum Büschenfelde K H
Z Gastroenterol. 1978 Dec;16(12):739-47.
In vivo fixation of immunoglobulin and C3 onto the hepatocellular membrane of patients with Crohn's disease was studied by immunofluorescence. IgG binding was observed in 93% of patients with disease activity but not in patients without disease activity. C3 binding was present in 21% of patients with active disease. IgA binding was found in approximately one half of all patients with Crohn's disease irrespective of disease activity. The immunoglobulin and C3 binding to hepatocytes was not correlated with histopathological findings. Circulating immune complexes were detected with the Raji cell assay in approximately one half of all patients with Crohn's disease and showed no correlation to disease activity. The following conclusions can be derived from these studies: 1. The in vivo fixed IgG represents immune complexes, which is eliminated by the liver as part of the physiological clearance function. 2. These aggregates bind to hepatocytes predominantly via IgGFc receptors. 3. Hepatocellular bound IgG and C3 show no correlation to histopathological liver alterations. 4. In vivo bound hepatocellular IgG aggregates have different properties than the immune complexes detected by the Raji cell assay.
通过免疫荧光研究了免疫球蛋白和C3在克罗恩病患者肝细胞膜上的体内固定情况。在93%有疾病活动的患者中观察到IgG结合，但在无疾病活动的患者中未观察到。21%有活动性疾病的患者存在C3结合。在所有克罗恩病患者中，约一半患者发现有IgA结合，与疾病活动无关。免疫球蛋白和C3与肝细胞的结合与组织病理学结果无关。在所有克罗恩病患者中，约一半患者通过Raji细胞试验检测到循环免疫复合物，且与疾病活动无关。从这些研究中可以得出以下结论：1. 体内固定的IgG代表免疫复合物，肝脏作为生理清除功能的一部分将其清除。2. 这些聚集体主要通过IgGFc受体与肝细胞结合。3. 肝细胞结合的IgG和C3与肝脏组织病理学改变无关。4. 体内结合的肝细胞IgG聚集体与通过Raji细胞试验检测到的免疫复合物具有不同的特性。
