Gustafsson B
Int Arch Allergy Appl Immunol. 1980;63(2):121-8. doi: 10.1159/000232617.
Actively sensitized rat mast cells were stimulated to secretion in vitro by means of an anaphylactic IgE antigen reaction or with polymyxin B. Release of 5-hydroxytryptamine (5-HT) and heparin from the cells and from individual granules, extruded or displaced from stimulated cells with micromanipulation, was quantitated cytofluorometrically. Several similarities in action between the two secretagogues were found. All extruded and some intracellular granules from stimulated cells lacked 5-HT, and no heparin was released from the granules. Heparin secretion from mast cells was due to granule extrusion alone. Further, the proportions between 5-HT and heparin released from mast cells indicated that amine release, too, is mainly, but not exclusively, associated with overt granule exocytosis. The results suggested that the first extruded granules are the richest in both 5-HT and heparin. Antigen-induced secretion was not as vigorous as that induced by polymyxin and, unlike the latter, followed after a short lag of about 20 sec.
通过过敏IgE抗原反应或用多粘菌素B在体外刺激主动致敏的大鼠肥大细胞使其分泌。用细胞荧光测定法定量从细胞以及通过显微操作从受刺激细胞挤出或移位的单个颗粒中5-羟色胺（5-HT）和肝素的释放量。发现两种促分泌剂在作用上有几个相似之处。受刺激细胞中所有挤出的和一些细胞内颗粒都缺乏5-HT，并且颗粒中没有肝素释放。肥大细胞的肝素分泌仅归因于颗粒挤出。此外，从肥大细胞释放的5-HT和肝素之间的比例表明，胺的释放也主要但并非唯一地与明显的颗粒胞吐作用相关。结果表明，最初挤出的颗粒中5-HT和肝素含量最丰富。抗原诱导的分泌不如多粘菌素诱导的分泌强烈，并且与后者不同，抗原诱导的分泌在约20秒的短暂延迟后发生。
