Nilsson L A, Björck L, Elwing H, Ouchterlony O
Int Arch Allergy Appl Immunol. 1980;63(3):294-302. doi: 10.1159/000232639.
Thin layer immunoassay (TIA), a solid phase immunoassay based on a technically simple visualization method, was adapted for quantitative determination of rheumatoid factor (RF) in blood serum. The influence of various factors of possible significance for accurate quantitation of RF by TIA was studied. Comparison between TIA and the Waaler-Rose test for determination of RF was performed on 335 patient sera. The results obtained by the two methods agreed reasonably well. Preliminary experiments were also performed concerning the use of TIA for differentiation of RF with regard to the immunoglobulin class. It is concluded that, because of its simplicity and screening capacity, TIA could be an attractive method for detection and quantiation of RF.
薄层免疫测定法（TIA）是一种基于技术上简单的可视化方法的固相免疫测定法，适用于血清中类风湿因子（RF）的定量测定。研究了可能对通过TIA准确定量RF有重要意义的各种因素的影响。对335份患者血清进行了TIA与瓦勒-罗斯试验测定RF的比较。两种方法得到的结果相当吻合。还进行了关于使用TIA区分不同免疫球蛋白类别的RF的初步实验。结论是，由于其简单性和筛选能力，TIA可能是一种用于检测和定量RF的有吸引力的方法。
