Aziz Faisal, Kazmi Shahana Urooj
The Hormel Institute, University of Minnesota, Austin, MN 55912, USA.
Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, Karachi 75270, Pakistan.
Diagnostics (Basel). 2023 Jan 31;13(3):517. doi: 10.3390/diagnostics13030517.
is a causative agent of gastritis, gastroduodenal ulcers and gastric adenocarcinoma. The majority of -associated patients live in underdeveloped areas, facing the problem of lack of proper diagnostic facility. Hence, a simple and economical assay is required to handle the majority of gastric patients. Serum samples from gastroduodenal ulcers and gastritis patients were screened for infection by thin layer immunoassay. A polystyrene plate coated with sonicate whole cell antigen (10 µg/mL). Two-fold diluted patient's serum was allowed to react at 37 °C, incubated at 60 °C for 1 min over a water bath and the water condensation pattern for the antibody was recorded. ELISAs were used as reference assays to evaluate the efficacy of the developed thin layer immunoassay (TLI). Gastric patients' blood samples (62% male and 6% female) tested positive for , while age-wise, 15-25-year-old males (36%) and 65-75-year-old females (50%) showed the highest number of infections. TLI showed sensitivity (72-67%), specificity (100%), accuracy (94-69%) and κ value (0.493-0.357) in comparison with wELISA (Surface whole cell ELISA), sELISA (sonicate whole cell ELISA) and kELISA (commercial KIT ELISA). We conclude that thin layer immunoassay is a low cost, fast, simple and clinically reliable method for diagnosis at initial stages in patients in under-developed countries.
是胃炎、胃十二指肠溃疡和胃腺癌的致病因子。大多数相关患者生活在欠发达地区，面临缺乏适当诊断设施的问题。因此，需要一种简单且经济的检测方法来应对大多数胃病患者。通过薄层免疫测定法对胃十二指肠溃疡和胃炎患者的血清样本进行感染筛查。用超声破碎全细胞抗原（10μg/mL）包被聚苯乙烯板。将两倍稀释的患者血清在37°C下反应，在水浴中于60°C孵育1分钟，并记录抗体的水冷凝模式。酶联免疫吸附测定法（ELISA）用作参考检测方法，以评估所开发的薄层免疫测定法（TLI）的功效。胃病患者的血液样本（62%为男性，38%为女性）检测呈阳性，按年龄划分，15 - 25岁男性（36%）和65 - 75岁女性（50%）的感染人数最多。与全表面细胞ELISA（wELISA）、超声破碎全细胞ELISA（sELISA）和商业试剂盒ELISA（kELISA）相比，TLI显示出敏感性（72 - 67%）、特异性（100%）、准确性（94 - 69%）和κ值（0.493 - 0.357）。我们得出结论，薄层免疫测定法是一种低成本、快速、简单且临床可靠的方法，可用于欠发达国家患者的初步诊断。
需注意，原文中“6% female”可能有误，推测应为“38% female”，译文已按推测修改。
