Kubo K, Ishikura T, Fukagawa Y
J Antibiot (Tokyo). 1980 Jun;33(6):556-65. doi: 10.7164/antibiotics.33.556.
L-Amino acid acylase and D-amino acid acylase were stable below 50 degrees C, although the D-enzyme was more thermostable than the L-enzyme at higher temperatures. At 30 degrees C they showed the highest reaction velocity in phosphate buffer of pH 7.4. Hg++ and Cu++ severely inactivated their activity. Activation by Co++ was observed on L-amino acid acylase, but not on D-amino acid acylase. p-Chloromercuribenzoate inhibited both enzymes, whereas ethylenediamine tetraacetate was very inhibitory on L-amino acid acylase only. With N-acetyl- and N-chloroacetyl-amino acids as substrates, they were relatively stereo-specific. They acted as a peptidase on dipeptides and tripeptides. Although N-acetylglycine was attacked by the two enzymes, N-acetylglucosamine and N-acetylethanolamine were insusceptible. PS-5 was converted to NS-5 (deacetyl PS-5) by L-amino acid acylase as well as by D-amino acid acylase.
L-氨基酸酰化酶和D-氨基酸酰化酶在50摄氏度以下是稳定的，尽管在较高温度下D-酶比L-酶更耐热。在30摄氏度时，它们在pH 7.4的磷酸盐缓冲液中表现出最高的反应速度。Hg++和Cu++会严重使其活性失活。观察到Co++对L-氨基酸酰化酶有激活作用，但对D-氨基酸酰化酶没有激活作用。对氯汞苯甲酸抑制这两种酶，而乙二胺四乙酸仅对L-氨基酸酰化酶有很强的抑制作用。以N-乙酰基和N-氯乙酰基氨基酸为底物时，它们具有相对的立体特异性。它们对二肽和三肽起肽酶的作用。尽管N-乙酰甘氨酸会被这两种酶作用，但N-乙酰葡糖胺和N-乙酰乙醇胺不受影响。L-氨基酸酰化酶和D-氨基酸酰化酶都能将PS-5转化为NS-5（脱乙酰基PS-5）。
