Fukagawa Y, Kubo K, Ishikura T, Kouno K
J Antibiot (Tokyo). 1980 Jun;33(6):543-9. doi: 10.7164/antibiotics.33.543.
PS-5 was deacetylated to NS-5 (deacetylated PS-5) by l-amino acid acylase from porcine kidney and D-amino acid acylase from Streptomyces olivaceus but not by l-amino acid acylase from Aspergillus sp. Using PS-5, N-chloroacetyl-l-phenylalanine and N-chloroacetyl-D-valine as substrates, acylase producers were screened among facultative methanol-assimilating bacteria. Most of the microbes tested were active and could be classified into two groups of l-acylase producers and L-& D-acylase producers. Pseudomonas sp. 1158 which deacetylated the three substrates was chosen for further study. Cells of the bacterium entrapped in polyacrylamide gel and its acylase activities immobilized on DEAE-Sephadex were found to be useful for conversion of PS-5 to NS-5.
猪肾来源的L-氨基酸酰基转移酶和橄榄链霉菌来源的D-氨基酸酰基转移酶可将PS-5脱乙酰化为NS-5（脱乙酰化PS-5），但曲霉属来源的L-氨基酸酰基转移酶则不能。以PS-5、N-氯乙酰-L-苯丙氨酸和N-氯乙酰-D-缬氨酸为底物，在兼性甲醇同化细菌中筛选酰基转移酶产生菌。大多数测试微生物具有活性，可分为L-酰基转移酶产生菌和L-及D-酰基转移酶产生菌两组。选择能使三种底物脱乙酰化的假单胞菌属1158进行进一步研究。发现包埋在聚丙烯酰胺凝胶中的该细菌细胞及其固定在DEAE-葡聚糖上的酰基转移酶活性可用于将PS-5转化为NS-5。
