Jones G S, Van Dyke K, Castranova V
J Cell Physiol. 1980 Sep;104(3):425-31. doi: 10.1002/jcp.1041040315.
Viable cell samples containing 93% pure granulocytes were obtained from human blood using the techniques of dextran sedimentation followed by centrifugal elutriation. The resting transmembrane potential (Em) of human granulocytes was estimated using the fluorescent lipophilic cation, Di-S-C3(5), from the null point for potassium--i.e., the external K concentration at which there is no change in Em in response to valinomycin (a K ionophore). The Em of human granulocytes, as calculated from the Nernst potential for K at the null point, is approximately -100 mV. Data indicate that this large transmembrane potential is due in part to the presence of an electrogenic Na-K pump in human granulocytes which is stimulated by external potassium and inhibited by ouabain.
采用葡聚糖沉降后离心淘洗技术，从人血液中获取了含93%纯粒细胞的活细胞样本。使用荧光亲脂性阳离子Di-S-C3(5)，从钾的零点（即外部钾浓度，此时膜电位（Em）不会因缬氨霉素（一种钾离子载体）而改变）来估算人粒细胞的静息跨膜电位（Em）。根据零点时钾的能斯特电位计算，人粒细胞的Em约为 -100 mV。数据表明，这种大的跨膜电位部分归因于人粒细胞中存在一种电生性钠钾泵，该泵受外部钾刺激并被哇巴因抑制。
