Henderson L M, Chappell J B, Jones O T
Department of Biochemistry, Medical School, University of Bristol, U.K.
Biochem J. 1987 Sep 1;246(2):325-9. doi: 10.1042/bj2460325.
The membrane potential of cytoplasts, derived from human neutrophils, was depolarized by the activation of the superoxide-generating NADPH-dependent oxidase. The extent of the depolarization was inhibited by diphenylene iodonium and was therefore due directly to the activity of the oxidase, which must be electrogenic. The extent of the depolarization was influenced by alteration of the delta pH across the cytoplast membrane, indicating that the outward translocation of H+ eventually compensates for superoxide generation. The depolarization of the potential is enhanced by Cd2+, a blocker of H+ currents, suggesting that the compensatory movement is via an H+ channel.
源自人类中性粒细胞的胞质体的膜电位，通过超氧化物生成的NADPH依赖性氧化酶的激活而发生去极化。去极化的程度受到二苯基碘鎓的抑制，因此直接归因于氧化酶的活性，该氧化酶必定是生电的。跨胞质体膜的δpH的改变影响了去极化的程度，表明H⁺的外向转运最终补偿了超氧化物的生成。H⁺电流阻滞剂Cd²⁺增强了电位的去极化，表明补偿性运动是通过H⁺通道进行的。
