用3H-NSP（N-琥珀酰亚胺基-[2,3-3H]丙酸酯）进行蛋白质标记。
Protein labelling with 3H-NSP (N-succinimidyl-[2,3-3H]propionate).
作者信息
Müller G H
出版信息
J Cell Sci. 1980 Jun;43:319-28. doi: 10.1242/jcs.43.1.319.
PMID:7419623
Abstract
A tritium compound with low molecular weight and diameter, N-succinimidyl-[2,3-3H]-propionate (3H-NSP), was used to label histones and nonhistone proteins from calf thymus, and nuclear and total salivary gland proteins from larvae of the midge Chironomus thummi. Labelled proteins were visualized after electrophoresis in 10% polyacrylamide slab gels by fluorography. The method is much more sensitive than staining procedures, allowing to discern approx. 0.1 microgram protein.
摘要
一种低分子量和直径的氚化合物，N-琥珀酰亚胺基-[2,3-³H]-丙酸酯（³H-NSP），被用于标记来自小牛胸腺的组蛋白和非组蛋白，以及来自摇蚊幼虫唾液腺的核蛋白和总蛋白。在10%聚丙烯酰胺平板凝胶中进行电泳后，通过荧光自显影对标记的蛋白质进行可视化。该方法比染色程序灵敏得多，能够辨别约0.1微克的蛋白质。
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