用N-琥珀酰亚胺基[2,3-³H]丙酸对α-银环蛇毒素进行氚化。一种用于标记蛋白质的有用试剂。
Tritiation of alpha-bungarotoxin with N-succinimidyl [2,3-3H]propionate. A useful reagent for labelling proteins.
作者信息
Dolly J O, Nockles E A, Lo M M, Barnard E A
出版信息
Biochem J. 1981 Mar 1;193(3):919-23. doi: 10.1042/bj1930919.
Abstract
Mono[3H]propionyl-alpha-bungarotoxin, prepared with N-succinimidyl [2,3-3H]propionate (sp. radioactivity 50 Ci/mmol) and purified to homogeneity by electrofocusing, retains its biological activity and stability. Rate constants for its binding to acetylcholine receptor were 4.4-fold lower than for unlabelled toxin; no dissociation was detectable. Analysis of enzymic digests of toxin showed 3H is located mainly of entirely in epsilon-propionyl-lysine.
摘要
用N-琥珀酰亚胺基[2,3-³H]丙酸（比放射性50 Ci/mmol）制备并通过电聚焦纯化至同质的单[³H]丙酰-α-银环蛇毒素保留了其生物活性和稳定性。其与乙酰胆碱受体结合的速率常数比未标记的毒素低4.4倍；未检测到解离。毒素酶解消化分析表明³H主要或完全位于ε-丙酰赖氨酸中。
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