Hoeldtke R D, Stetson P L
J Clin Endocrinol Metab. 1980 Oct;51(4):810-15. doi: 10.1210/jcem-51-4-810.
A non-invasive method is described for estimating dopamine beta-hydroxylase (DBH) activity in sympathetic neurons. Our assay is based on the measurement of the in vivo rate of tritiated water (THO) release from dopamine specifically labeled in the beta-position ([beta-3H]DA). In validation of this method, we have establsihed in human subjects that 1) THO release from [beta-3H]DA correlates with [beta-3H]norepinephrine metabolite excretion, and 2) partial inhibition of DBH with disulfiram (5.5 mg/kg for 4 days) causes parallel decreases in these two indices of dopamine beta-hydroxylation. Since there is a strong correlation (r = 0.89; P < 0.05) between the effect of disulfiram on THO release and the effect of the drug on [3H]-norepinephrine metabolite excretion, our data indicate that the rate at which THO is liberated from [beta-3H]DA into the total body water can be used as an in vivo index of DBH activity.
本文描述了一种用于评估交感神经元中多巴胺β-羟化酶（DBH）活性的非侵入性方法。我们的检测方法基于对从β位特异性标记的多巴胺（[β-3H]DA）中释放的氚水（THO）的体内释放速率的测量。在对该方法的验证中，我们在人类受试者中证实：1）[β-3H]DA释放的THO与[β-3H]去甲肾上腺素代谢产物排泄相关；2）用双硫仑（5.5mg/kg，持续4天）部分抑制DBH会导致多巴胺β-羟化这两个指标平行下降。由于双硫仑对THO释放的影响与药物对[3H]-去甲肾上腺素代谢产物排泄的影响之间存在很强的相关性（r = 0.89；P < 0.05），我们的数据表明，THO从[β-3H]DA释放到全身水中的速率可用作DBH活性的体内指标。
