Latman N S
J Exp Zool. 1980 Jun;212(3):305-11. doi: 10.1002/jez.1402120302.
A system for the in vitro perfusion of bovine parathyroid glands was developed. The glands were perfused at 1 ml/minute with oxygenated Hanks balanced salt solution buffered with sodium bicarbonate to a pH of 7.4 at 37 degrees C. These glands remained functionally stable for seven to eight hours. The secretory rate of immunoreactive parathyroid hormone varied inversely with the calcium concentration of 3.0 to 6.0 mg/100 and became nonsuppressible at calcium concentrations above 6.5 mg/100 ml. Therefore, the glands are thought to be responsive to stimuli. A loss of functional stability can usually be detected by an increase in perfusion pressure. The samples of effluent containing immunoreactive parathyroid hormone remained stable (immunoreactively) for six months when stored at -70 degrees C. This system provides a valid method for monitoring dynamic, minute-to-minute changes in parathyroid secretory activity.
开发了一种用于牛甲状旁腺体外灌注的系统。腺体以每分钟1毫升的速度用含碳酸氢钠缓冲至pH值为7.4的含氧汉克斯平衡盐溶液在37摄氏度下进行灌注。这些腺体在功能上保持稳定七至八小时。免疫反应性甲状旁腺激素的分泌率与钙浓度在3.0至6.0毫克/100毫升之间呈反比，并且在钙浓度高于6.5毫克/100毫升时变得不可抑制。因此，认为这些腺体对刺激有反应。功能稳定性的丧失通常可通过灌注压力的增加来检测。含有免疫反应性甲状旁腺激素的流出物样本在-70摄氏度下储存时在免疫反应性方面保持稳定六个月。该系统为监测甲状旁腺分泌活动的动态、每分钟变化提供了一种有效的方法。
