体外有功能的牛甲状旁腺直接释放甲状旁腺激素片段。
Direct release of parathyroid hormone fragments from functioning bovine parathyroid glands in vitro.
作者信息
Hanley D A, Takatsuki K, Sultan J M, Schneider A B, Sherwood L M
出版信息
J Clin Invest. 1978 Dec;62(6):1247-54. doi: 10.1172/JCI109245.
Abstract
To determine the origin of circulating parathyroid hormone fragments, hormonal peptides released from bovine parathyroid tissue in a physiologically responsive in vitro "perifusion" system were analyzed by gel exclusion chromatography and region-specific radioimmunoassays. When exposed to low Ca++, the tissue released large quantities of intact hormone (parathyroid hormone 1--84) as well as amino- and carboxyl-terminal fragments. Fragments of the hormone were also released when the tissue was exposed to high Ca++, but the carboxyl fragments comprised a much greater proportion of the hormonal peptides released. Control experiments indicated that fragmentation of the hormone occurred within the gland and not after it was secreted. These experiments provide direct evidence, therefore, that release of fragments from the parathyroid gland may contribute to the immunologic heterogeneity of the hormone in the circulation.
摘要
为了确定循环甲状旁腺激素片段的来源，通过凝胶排阻色谱法和区域特异性放射免疫测定法，对在生理反应性体外“灌流”系统中从牛甲状旁腺组织释放的激素肽进行了分析。当暴露于低钙离子浓度时，该组织会释放大量完整激素（甲状旁腺激素1-84）以及氨基末端和羧基末端片段。当组织暴露于高钙离子浓度时，也会释放激素片段，但羧基片段在释放的激素肽中所占比例要大得多。对照实验表明，激素的片段化发生在腺体内部，而非分泌之后。因此，这些实验提供了直接证据，表明甲状旁腺释放片段可能导致循环中该激素的免疫异质性。
相似文献
1
Direct release of parathyroid hormone fragments from functioning bovine parathyroid glands in vitro.体外有功能的牛甲状旁腺直接释放甲状旁腺激素片段。
J Clin Invest. 1978 Dec;62(6):1247-54. doi: 10.1172/JCI109245.
2
Calcium-dependent release of carboxyl-terminal fragments of parathyroid hormone by hyperplastic human parathyroid tissue in vitro.
J Clin Endocrinol Metab. 1986 Nov;63(5):1075-9. doi: 10.1210/jcem-63-5-1075.
3
Responsiveness of neoplastic and hyperplastic parathyroid tissues to calcium in vitro.肿瘤性和增生性甲状旁腺组织在体外对钙的反应性。
J Clin Invest. 1978 Aug;62(2):436-50. doi: 10.1172/JCI109145.
4
A direct effect in vitro of phosphate on PTH release from bovine parathyroid tissue slices but not from dispersed parathyroid cells.磷酸盐对牛甲状旁腺组织切片中甲状旁腺激素（PTH）释放有直接体外效应，但对分散的甲状旁腺细胞则没有。
Nephrol Dial Transplant. 1996 Sep;11(9):1762-8.
5
Structural analysis of parathormone fragments elaborated by cells cultured from a hyperplastic human parathyroid gland.
Bone Miner. 1986 Feb;1(1):41-50.
6
The secretion of parathyroid hormone and its fragments from dispersed cells of adenomatous parathyroid tissue at different calcium concentrations.
Exp Clin Endocrinol. 1986 Nov;88(1):101-8. doi: 10.1055/s-0029-1210582.
7
The effects of phorbol myristate acetate on the intracellular degradation of bovine parathyroid hormone.佛波醇肉豆蔻酸酯乙酸盐对牛甲状旁腺激素细胞内降解的影响。
Endocrinology. 1991 Apr;128(4):1863-8. doi: 10.1210/endo-128-4-1863.
8
Immunoheterogeneity of parathyroid hormone in venous effluent serum from hyperfunctioning parathyroid glands.功能亢进甲状旁腺静脉流出血清中甲状旁腺激素的免疫异质性
J Clin Invest. 1977 Dec;60(6):1367-75. doi: 10.1172/JCI108897.
9
A "carboxyl terminal" clinical radioimmunoassay for parathyroid hormone with apparent recognition preference for the intact hormone.一种对甲状旁腺激素具有明显对完整激素识别偏好的“羧基末端”临床放射免疫测定法。
J Immunoassay. 1985;6(3):245-59. doi: 10.1080/01971528508063032.
10
Calcium-dependent intracellular degradation of parathyroid hormone: a possible mechanism for the regulation of hormone stores.甲状旁腺激素的钙依赖性细胞内降解：激素储存调节的一种可能机制。
Endocrinology. 1975 Aug;97(2):431-41. doi: 10.1210/endo-97-2-431.
引用本文的文献
1
The Pathophysiology of Secondary Hyperparathyroidism and the Consequences of Uncontrolled Mineral Metabolism in Chronic Kidney Disease: The Role of COSMOS.继发性甲状旁腺功能亢进的病理生理学及慢性肾脏病中矿物质代谢失控的后果：COSMOS的作用
NDT Plus. 2008 Jan;1(Suppl 1):i2-i6. doi: 10.1093/ndtplus/sfm037.
2
Parathyroid hormone assay: problems and opportunities.甲状旁腺激素检测：问题与机遇
Pediatr Nephrol. 2007 Oct;22(10):1651-4. doi: 10.1007/s00467-007-0508-0. Epub 2007 May 22.
3
Reference range for serum parathyroid hormone.血清甲状旁腺激素的参考范围。
Endocr Pract. 2006 Mar-Apr;12(2):137-44. doi: 10.4158/EP.12.2.137.
4
"Asymptomatic" and symptomatic primary hyperparathyroidism.无症状性和有症状性原发性甲状旁腺功能亢进症。
Clin Investig. 1993 Jul;71(7):505-18. doi: 10.1007/BF00208472.
5
Immunoheterogeneity of parathyroid hormone pre- and postoperatively in primary hyperparathyroidism.原发性甲状旁腺功能亢进症患者甲状旁腺激素术前及术后的免疫异质性
Langenbecks Arch Chir. 1995;380(2):119-24. doi: 10.1007/BF00186419.
6
Effects of hepatectomy, nephrectomy, and nephrectomy/uremia on the metabolism of parathyroid hormone in the rat.肝切除术、肾切除术以及肾切除术/尿毒症对大鼠甲状旁腺激素代谢的影响。
J Clin Invest. 1981 Feb;67(2):439-48. doi: 10.1172/JCI110052.
7
Regulation of parathyroid hormone secretion in vitro and in vivo.甲状旁腺激素分泌在体外和体内的调节。
Calcif Tissue Int. 1982 Jul;34(4):313-6. doi: 10.1007/BF02411259.
8
Effects of magnesium ion on parathyroid hormone secretion in vitro.镁离子对体外甲状旁腺激素分泌的影响。
Calcif Tissue Int. 1980;32(3):201-6. doi: 10.1007/BF02408542.
9
In vitro perifusion for the study of parathyroid hormone secretion: effects of extracellular calcium concentration and beta-adrenergic regulation on bovine parathyroid hormone secretion in vitro.用于甲状旁腺激素分泌研究的体外灌流：细胞外钙浓度和β-肾上腺素能调节对体外牛甲状旁腺激素分泌的影响
Calcif Tissue Int. 1980;32(1):19-27. doi: 10.1007/BF02408518.
10
Factors that influence the assessment of parathyroid graft function.影响甲状旁腺移植功能评估的因素。
Ann Surg. 1984 Feb;199(2):192-6. doi: 10.1097/00000658-198402000-00011.
本文引用的文献
1
Parathyroid Hormone in Human Plasma: IMMUNOCHEMICAL CHARACTERIZATION AND BIOLOGICAL IMPLICATIONS.人血浆中的甲状旁腺激素：免疫化学特征及生物学意义。
J Clin Invest. 1972 Dec;51(12):3163-72. doi: 10.1172/JCI107143.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
ANTIBODIES TO BRADYKININ AND ANGIOTENSIN: A USE OF CARBODIIMIDES IN IMMUNOLOGY.抗缓激肽和血管紧张素抗体：碳二亚胺在免疫学中的一种应用
Science. 1964 Jun 12;144(3624):1344-6. doi: 10.1126/science.144.3624.1344.
4
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
Biochem J. 1963 Oct;89(1):114-23. doi: 10.1042/bj0890114.
5
Immunogenicity of a series of alpha,N-DNP-L-lysines.一系列α，N-二硝基苯-L-赖氨酸的免疫原性
Biochemistry. 1965 Aug;4(8):1638-45. doi: 10.1021/bi00884a028.
6
Immunochemical heterogeneity of parathyroid hormone in plasma.血浆中甲状旁腺激素的免疫化学异质性
J Clin Endocrinol Metab. 1968 Jul;28(7):1037-47. doi: 10.1210/jcem-28-7-1037.
7
Dynamics of parathyroid hormone secretion in vitro.甲状旁腺激素体外分泌动力学
Am J Med. 1971 May;50(5):650-7. doi: 10.1016/0002-9343(71)90120-3.
8
Regulation of parathyroid hormone secretion in vitro: quantitative aspects of calcium and magnesium ion control.甲状旁腺激素分泌的体外调节：钙和镁离子控制的定量方面。
Endocrinology. 1971 Jun;88(6):1477-82. doi: 10.1210/endo-88-6-1477.
9
Isolation and characterization of the bovine parathyroid isohormones.牛甲状旁腺同工激素的分离与特性分析。
Biochemistry. 1971 Jul 6;10(14):2779-87. doi: 10.1021/bi00790a020.
10
A method for producing specific antisera with small doses of immunogen.一种用小剂量免疫原制备特异性抗血清的方法。
J Clin Endocrinol Metab. 1971 Dec;33(6):988-91. doi: 10.1210/jcem-33-6-988.
Zotero 插件