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腺病毒DNA复制的起始

Initiation of adenovirus DNA replication.

作者信息

Reiter T, Fütterer J, Weingärtner B, Winnacker E L

出版信息

J Virol. 1980 Sep;35(3):662-71. doi: 10.1128/JVI.35.3.662-671.1980.

DOI:10.1128/JVI.35.3.662-671.1980
PMID:7420538
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC288860/
Abstract

In an attempt to study the mechanism of initiation of adenovirus DNA replication, an assay was developed to investigate the pattern of DNA synthesis in early replicative intermediates of adenovirus DNA. By using wild-type virus-infected cells, it was possible to place the origin of adenovirus type 2 DNA replication within the terminal 350 to 500 base pairs from either of the two molecular termini. In addition, a variety of parameters characteristic of adenovirus DNA replication were compared with those obtained in a soluble nuclear extract competent for viral DNA replication. It was observed that in vitro DNA replication, which is dependent on the exogenously added viral DNA-protein complex as its optimal template, occurs in a manner apparently indistinguishable from the situation in virus-infected cells. This includes the presence of proteinaceous material on the molecular termini of newly initiated viral DNA.

摘要

为了研究腺病毒DNA复制起始的机制，开发了一种测定方法来研究腺病毒DNA早期复制中间体中的DNA合成模式。通过使用野生型病毒感染的细胞，有可能将腺病毒2型DNA复制的起始位点定位在两个分子末端中任一端的末端350至500个碱基对范围内。此外，将腺病毒DNA复制的各种特征参数与在能够进行病毒DNA复制的可溶性核提取物中获得的参数进行了比较。据观察，体外DNA复制依赖于外源添加的病毒DNA - 蛋白质复合物作为其最佳模板，其发生方式与病毒感染细胞中的情况明显无法区分。这包括新起始的病毒DNA分子末端存在蛋白质物质。

相似文献

1
Initiation of adenovirus DNA replication.腺病毒DNA复制的起始
J Virol. 1980 Sep;35(3):662-71. doi: 10.1128/JVI.35.3.662-671.1980.
2
Adenovirus DNA replication in vitro: a protein linked to the 5' end of nascent DNA strands.腺病毒DNA的体外复制：一种与新生DNA链5'末端相连的蛋白质。
J Virol. 1981 Jan;37(1):139-47. doi: 10.1128/JVI.37.1.139-147.1981.
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Replication of adenovirus DNA-protein complex with purified proteins.用纯化蛋白对腺病毒DNA-蛋白质复合物进行复制。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):884-8. doi: 10.1073/pnas.78.2.884.
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Multiple rounds of adenovirus DNA synthesis in vitro.体外进行多轮腺病毒DNA合成。
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Studies on the mechanism of replication of adenovirus DNA. V. The location of termini of replication.
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[Synthesis of adenovirus SA7 and Ad5 DNA in extracts from the nuclei of green monkey kidney cells].[在绿猴肾细胞核提取物中腺病毒SA7和Ad5 DNA的合成]
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Kinetics of adenovirus DNA replication II. Initiation of adenovirus DNA replication.腺病毒DNA复制的动力学II. 腺病毒DNA复制的起始
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引用本文的文献

1
A size analysis of the adenovirus replicon.腺病毒复制子的大小分析。
EMBO J. 1984 Feb;3(2):333-7. doi: 10.1002/j.1460-2075.1984.tb01807.x.
2
Nucleotide sequence of the early genes 3 and 4 of bacteriophage phi 29.噬菌体φ29早期基因3和4的核苷酸序列。
Nucleic Acids Res. 1982 Oct 11;10(19):5785-98. doi: 10.1093/nar/10.19.5785.
3
Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage phi 29.枯草芽孢杆菌噬菌体 phi 29 DNA 末端的核苷酸序列。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1446-50. doi: 10.1073/pnas.78.3.1446.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Purification of polyoma virus.多瘤病毒的纯化
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A circula DNA-protein complex from adenoviruses.一种来自腺病毒的环状DNA-蛋白质复合物。
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Studies on the mechanism of replication of adenovirus DNA. I. The effect of hydroxyurea.腺病毒DNA复制机制的研究。I. 羟基脲的作用
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Synthesis of type 2 adenovirus DNA in the presence of cycloheximide.在环己酰亚胺存在的情况下2型腺病毒DNA的合成。
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Structure and composition of the adenovirus type 2 core.2型腺病毒核心的结构与组成
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Adenovirus type 2 DNA replication. I. Evidence for discontinuous DNA synthesis.腺病毒2型DNA复制。I. 间断性DNA合成的证据。
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Cleavage maps of DNA from adenovirus types 2 and 5 by restriction endonucleases EcoRI and HpaI.用限制性内切酶EcoRI和HpaI对2型和5型腺病毒的DNA进行切割图谱分析。
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