一种新型噬菌体Psp231a感染菜豆假单胞菌HB10Y的特性

Characteristics of a new bacteriophage, Psp231a, infecting Pseudomonas phaseolicola HB10Y.

作者信息

Taylor W D, DeLuca N, Vollherbst K, Doman T, d'Amato T, Snipes W

出版信息

J Virol. 1980 Sep;35(3):918-23. doi: 10.1128/JVI.35.3.918-923.1980.

DOI:10.1128/JVI.35.3.918-923.1980

PMID:7420544

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC288885/

Abstract

Bacteriophage Psp231a infects Pseudomonas phaseolicola, strain HB10Y, which is the host cell for the enveloped bacteriophage phi 6. This paper describes the biophysical characteristics of Psp231a and the physical properties of its nucleic acid. In electron micrographs the virion appears as an icosahedral structure, approximately 55 nm in diameter, with a short tail. The virion density is 1.48 g/cm3 in CsCl, and the sedimentation coefficient is approximately 407S. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of 12 polypeptides ranging in molecular weight from 5,000 to 117,000. The nucleic acid of Psp231a is linear, double-stranded DNA of molecular weight 28 X 10(6). Its density in CsCl is 1.716 g/cm3, and its sedimentation coefficient in 3 M CsCl is 20.0S, corresponding to an S020,W of 34S.

摘要

噬菌体Psp231a感染菜豆假单胞菌HB10Y菌株，该菌株是包膜噬菌体phi 6的宿主细胞。本文描述了Psp231a的生物物理特性及其核酸的物理性质。在电子显微镜照片中，病毒粒子呈现为二十面体结构，直径约55纳米，带有短尾。在氯化铯中病毒粒子密度为1.48克/立方厘米，沉降系数约为407S。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示存在12种分子量在5000至117000之间的多肽。Psp231a的核酸是线性双链DNA，分子量为28×10⁶。其在氯化铯中的密度为1.716克/立方厘米，在3M氯化铯中的沉降系数为20.0S，对应S₀₂₀,W为34S。