噬菌体φW - 14 DNA的碱敏感性
Alkali lability of bacteriophage phi W-14 DNA.
作者信息
Lewis H A, Miller R C, Stone J C, Warren R A
出版信息
J Virol. 1975 Dec;16(6):1375-9. doi: 10.1128/JVI.16.6.1375-1379.1975.
Abstract
The molecular weight of bacteriophage phi W-14 DNA, determined by velocity sedimentation in neutral sucrose gradients, was 92 +/- 6 X 10(6). The DNA showed marked fragmentation in alkaline sucrose gradients. This fragmentation was not a consequence of preexisting single-strand interruptions in the DNA, since thermal denaturation of DNA yielded intact single strands. The alpha-putrescinylthymine groups in phi W-14 DNA appeared to be labile; some, or parts of some, of these groups were cleaved from the DNA in alkali.
摘要
通过在中性蔗糖梯度中进行速度沉降测定，噬菌体φW - 14 DNA的分子量为92±6×10⁶ 。该DNA在碱性蔗糖梯度中显示出明显的片段化。这种片段化并非DNA中预先存在的单链中断所致，因为DNA的热变性产生了完整的单链。φW - 14 DNA中的α-腐胺基胸腺嘧啶基团似乎不稳定；这些基团中的一些或部分在碱中从DNA上被切割下来。
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