Pospelov V A, Svetlikova S B, Vorob'ev V I, Mikvits K U
Mol Biol (Mosk). 1980 Sep-Oct;14(5):1110-5.
Electron microscopic measurements of linker DNA were performed on chromatin from rat thymus cells and chicken erythrocytes which differ in biochemically determined DNA repeat sizes. The data obtained demonstrate that linker DNA is very heterogeneous in length and has size up to 100 nucleotide, pairs. Several discrete classes of linker DNA were revealed, they are multiples of 23--24 nucleotide pairs. The discrete and regular pattern of linker DNA classes allows to suggest that histone octamers have at least four zones of strong histone-histone interaction indispensable for tight contacts between nucleosomes. Provided the identity of packing of linker and core DNA, each turn of DNA should consist of about 95--100 nucleotide pairs. It gives a complete twist of nucleosomes along the axis of the chromatin thread through four zones of strong histone-histone interaction.
对大鼠胸腺细胞和鸡红细胞的染色质进行了连接DNA的电子显微镜测量，这两种细胞在生化测定的DNA重复序列大小上有所不同。获得的数据表明，连接DNA的长度非常不均一，大小可达100个核苷酸对。揭示了几种离散的连接DNA类别，它们是23 - 24个核苷酸对的倍数。连接DNA类别的离散且规则模式表明，组蛋白八聚体至少有四个强组蛋白-组蛋白相互作用区域，这些区域对于核小体之间的紧密接触是必不可少的。假设连接DNA和核心DNA的包装相同，DNA的每一圈应由约95 - 100个核苷酸对组成。这通过四个强组蛋白-组蛋白相互作用区域使核小体沿着染色质丝轴完全扭转。
