Pittilo R M, Ball S J
Parasitology. 1980 Aug;81(1):115-22. doi: 10.1017/s0031182000055086.
Oocyst wall formation in Eimeria maxima was studied during the macrogamete stage in intestinal cells of the chick and in unsporulated oocysts isolated from faeces. The outer of the 2 membranes bounding the mature macrogamete separated from the surface but remained as a veil surrounding the developing oocyst throughout the whole intracellular process. Wall-forming bodies Type I were initially applied to the limiting membrane of the zygote cytoplasm; a layer of material similar to their contents was then formed around the zygote. As this occurred a new double membrane was formed surrounding the oocyst cytoplasm. The outer wall layer was initially homogenous in appearance but later developed into 2 zones, an outer amorphous region and an inner osmiophilic region. The inner layer of the oocyst wall was formed from the contents of the wall-forming bodies Type II which dispersed between the outer wall and the limiting membranes of the oocyst cytoplasm. There was evidence of an additional membrane formed external to the outer wall. The outer membranes were not present around the wall of oocysts passed in the faeces of chicks, but the same wall zonation was evident, although the inner osmiophilic zone of the outer wall layer was markedly thinner in comparison with the same zone seen in the tissues.
在鸡的肠道细胞中的大配子阶段以及从粪便中分离出的未孢子化卵囊中，研究了巨型艾美耳球虫的卵囊壁形成过程。包围成熟大配子的两层膜中的外层与表面分离，但在整个细胞内过程中，仍作为一层面纱围绕着发育中的卵囊。I型壁状体最初附着在合子细胞质的限制膜上；然后在合子周围形成一层与其内容物相似的物质。当此情况发生时，围绕卵囊细胞质形成了一层新的双层膜。卵囊壁的外层最初外观均匀，但后来发展为两个区域，一个外部无定形区域和一个内部嗜锇区域。卵囊壁的内层由II型壁状体的内容物形成，这些内容物分散在外壁和卵囊细胞质的限制膜之间。有证据表明在外壁外部形成了一层额外的膜。在雏鸡粪便中排出的卵囊壁周围没有外层膜，但相同的壁分区很明显，尽管与在组织中看到的同一区域相比，外壁层的内部嗜锇区明显更薄。
