Michael E
Z Parasitenkd. 1978 Nov 27;57(3):221-8. doi: 10.1007/BF00928035.
The formation and final structure of the oocyst wall of Eimeria acervulina is described, based on a detailed electron microscope study of the maturing oocysts. After fertilization of the macrogametocyte the wall-forming bodies of type I progressively undergo disaggregation into smaller bodies and eventually move into spaces left by the pellicular membranes of the zygote, which simultaneously separate and elevate away from the zygote cytoplasm to form the outer layer of the oocyst wall. A newly formed membrane separates this layer from the cytoplasm. Following the formation of the outer layer, another membrane separates and elevates away from the cytoplasm, and the wall-forming bodies of type II, which by now have migrated to the periphery, move into the spaces and fuse together to form the inner layer of the oocyst wall. A newly formed membrane separates this layer from the cytoplasm. The wall of the young oocyst thus consists of two membrane-bound layers of approximately similar thickness; the outer layer being osmiophilic whilst the inner one is paler. An overlaying membrane covers the oocyst. The surface of the oocyst wall was smooth in appearance when viewed with the scanning electron microscope.
基于对成熟卵囊的详细电子显微镜研究，描述了堆型艾美耳球虫卵囊壁的形成和最终结构。大配子细胞受精后，I型壁形成体逐渐解体为较小的体，最终进入合子表膜留下的空间，合子表膜同时分离并从合子细胞质中升起，形成卵囊壁的外层。一层新形成的膜将这一层与细胞质分隔开。外层形成后，另一层膜从细胞质中分离并升起，此时已迁移到外周的II型壁形成体进入这些空间并融合在一起，形成卵囊壁的内层。一层新形成的膜将这一层与细胞质分隔开。年轻卵囊的壁因此由两层厚度大致相似的膜结合层组成；外层嗜锇，而内层较淡。一层覆盖膜覆盖着卵囊。用扫描电子显微镜观察时，卵囊壁表面外观光滑。
