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蛋白A血凝试验,一种快速鉴定金黄色葡萄球菌的可靠方法(作者译)

[Protein-A-hemagglutination test, a reliable method for rapid identification of S. aureus (author's transl)].

作者信息

Essers L, Radebold K

出版信息

Zentralbl Bakteriol A. 1980;247(2):170-6.

PMID:7424245
Abstract

369 staphylococcal strains isolated from clinical material were examined for tubeagglutination with sensitized sheep red cells in a standardized assay to study its reliability for routine identification of S. aureus. Colonies isolated from blood agar plates correlated in 99.5% with the (optimized) coagulase reaction. The test is easily to perform and results can be read after 2 hours, whereas the reference methods coagulase, hyaluronidase and deoxyribonuclease took as much as 24 h. The reliability of these tests is discussed.

摘要

对从临床材料中分离出的369株葡萄球菌菌株进行检测,采用标准化试验用致敏绵羊红细胞进行试管凝集试验,以研究其在常规鉴定金黄色葡萄球菌方面的可靠性。从血琼脂平板上分离出的菌落与(优化后的)凝固酶反应的相关性为99.5%。该试验易于操作,2小时后即可读取结果,而作为参考方法的凝固酶、透明质酸酶和脱氧核糖核酸酶检测则需要长达24小时。文中讨论了这些检测方法的可靠性。

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