Geraedts J P, Weber R F, Kerkhofs H, Leeksma C H
Acta Med Scand. 1980;207(6):447-54.
Out of 151 patients with preleukemic syndrome, bone marrow chromosome studies were carried out in 88 during the preleukemic phase and in 10 after blastic transformation. Out of 54 cases studied without banding techniques, 13 (24%) were abnormal, while 17 (50%) out of 34 banded cases showed abnormalities. This highly significant increase in yield of aberrations was not restricted to structural abnormalities. During the preleukemic phase of the disease, only 5 of the abnormal patients had no normal metaphases in their bone marrow. Four types of chromosome aberrations were observed more than once: -Y, +8, del 5q and del F or del 20q. They are all frequently observed in myeloproliferative disorders. After blastic transformation, 15 out of 19 patients were abnormal and the abnormalities were more complex. It seems, therefore, that a qualitative and quantitative difference exists between this group of patients and the published series of patients with ANLL. Small abnormal cell lines with the same chromosome abnormalities as in the bone marrow were observed in PHA-stimulated blood cultures of 9 patients. Unstimulated cultures of the same blood sample did not show any mitosis. It is suggested that small subpopulations of lymphocytes arose from the same pluripotent stem cell as the leukemic myelogenous cells, although there may be other explanations.
在151例白血病前期综合征患者中，88例在白血病前期阶段进行了骨髓染色体研究，10例在原始细胞转化后进行了研究。在54例未采用显带技术研究的病例中，13例（24%）异常，而在34例采用显带技术研究的病例中，17例（50%）显示异常。这种畸变检出率的显著提高不仅限于结构异常。在疾病的白血病前期阶段，异常患者中只有5例骨髓中没有正常中期分裂相。四种染色体畸变被多次观察到：-Y、+8、5q缺失和F缺失或20q缺失。它们在骨髓增殖性疾病中都经常被观察到。原始细胞转化后，19例患者中有15例异常，且异常情况更为复杂。因此，似乎该组患者与已发表的急性非淋巴细胞白血病（ANLL）患者系列之间存在质和量的差异。在9例患者的PHA刺激的血培养中观察到与骨髓中染色体异常相同的小异常细胞系。同一血样的未刺激培养未显示任何有丝分裂。有人提出，淋巴细胞的小亚群与白血病髓细胞起源于同一多能干细胞，尽管可能还有其他解释。
