Bell D R, Watson P D, Renkin E M
Am J Physiol. 1980 Oct;239(4):H532-H538. doi: 10.1152/ajpheart.1980.239.4.H532.
Exclusion of plasma albumin and fibrinogen from portions of the extravascular sucrose space in tissues of the hind paw of anesthetized dogs was studied by use of radioactive traces. Thirty hours after a bolus injection of the labeled proteins, tracer concentrations were measured in plasma, prenodal popliteal lymph, and samples of hind paw skin, tendon, muscle, and toe pad. In a few experiments, constant plasma levels of tracer sucrose were maintained for 2 h before terminal sampling. In all experiments a vascular volume marker was given 15 min before termination. A linear correlation was found between the amounts of extravascular protein tracers in the tissue samples and their concentrations in lymph from the same paw, as would be expected if their actual interstitial concentrations were closely related to their lymph concentrations. For albumin, the extravascular distribution volume calculated at lymph concentration was less than the sucrose space for all tissues except toe pad. For fibrinogen, the extravascular distribution volume was less than albumin and sucrose for all tissues except skin. Differences in pattern and degree of exclusion may reflect the differences in interstitial gel-phase composition and structure.
通过放射性示踪技术研究了麻醉犬后爪组织中部分血管外蔗糖空间对血浆白蛋白和纤维蛋白原的排除情况。在一次性注射标记蛋白30小时后，测量血浆、腘前淋巴结淋巴液以及后爪皮肤、肌腱、肌肉和趾垫样本中的示踪剂浓度。在一些实验中，在末次取样前2小时维持示踪剂蔗糖的血浆水平恒定。在所有实验中，在实验结束前15分钟给予血管容积标记物。组织样本中血管外蛋白示踪剂的量与其来自同一只爪子的淋巴液中的浓度之间存在线性相关性，这与如果它们的实际间质浓度与其淋巴液浓度密切相关时所预期的情况一致。对于白蛋白，在淋巴液浓度下计算出的血管外分布容积，除趾垫外，所有组织均小于蔗糖空间。对于纤维蛋白原，除皮肤外，所有组织的血管外分布容积均小于白蛋白和蔗糖。排除模式和程度的差异可能反映了间质凝胶相组成和结构的差异。
