Ottermann U
Anaesthesist. 1980 Apr;29(4):190-9.
On isolated normothermic and constant-pressure perfused rat-hearts cardioplegic haemoglobin-perfusion was compared with conventional cardioplegic perfusion without haemoglobin. The addition of 4% haemoglobin to the cardioplegic solution guarantees oxygen demand of the pharmacologically arrested heart at physiological PO2 (about 100 mm Hg) of the perfusion-solution, while conventional cardioplegic perfusion without haemoglobin needs arterial PO2 > 600 mm Hg and guarantees oxygen demand of the arrested normotherm rat-heart only under condition of sufficient coronary flow. The coronary flow during constant-pressure cardioplegic haemoglobin-perfusion was about 58% higher than during cardioplegic perfusion without haemoglobin. Interstitial oedema after perfusion for 1 hour was more intense after perfusion with the conventional cardioplegic solution than after cardioplegic haemoglobin-perfusion. Normothermic ischaemia of 20 minutes after perfusion with cardioplegic haemoglobin-solution was connected with a smaller drop of creatinephosphate and adenosinetriphosphate than after conventional cardioplegic perfusion, electron-optical preparations showed smaller changes of the structure. The use of cardioplegic haemoglobin-solutions in heart-surgery and the advantages of cardioplegic haemoglobinperfusion are discussed.
在离体正常体温、恒压灌注的大鼠心脏上，将含血红蛋白的心脏停搏灌注与不含血红蛋白的传统心脏停搏灌注进行了比较。在心脏停搏液中添加4%的血红蛋白可保证在灌注液生理氧分压（约100 mmHg）下，药物性停搏心脏的氧需求，而不含血红蛋白的传统心脏停搏灌注则需要动脉氧分压>600 mmHg，且仅在冠状动脉血流充足的情况下才能保证停搏的正常体温大鼠心脏的氧需求。恒压含血红蛋白心脏停搏灌注期间的冠状动脉血流比不含血红蛋白的心脏停搏灌注期间高约58%。灌注1小时后，用传统心脏停搏液灌注后的间质水肿比含血红蛋白心脏停搏灌注后的更严重。用含血红蛋白心脏停搏液灌注后20分钟的正常体温缺血与肌酸磷酸和三磷酸腺苷的下降幅度比传统心脏停搏灌注后更小，电子光学制剂显示结构变化更小。文中讨论了含血红蛋白心脏停搏液在心脏手术中的应用以及含血红蛋白心脏停搏灌注的优势。
