Sumikawa K, Amakata Y, Yoshikawa K, Kashimoto T, Izumi F
Anesthesiology. 1980 Nov;53(5):385-9. doi: 10.1097/00000542-198011000-00005.
The effects of halothane on the catecholamine release from chromaffin granules and the uptake of 14C-epinephrine into the granules were studied using isolated bovine adrenal medullary chromaffin granules. The granules were isolated with a Millipore filter, and were incubated for 10 min in an isotonic medium containing adenosine triphosphate (ATP)-Mg++. At halothane concentration of 0.4 mM or greater, the uptake of 14C-epinephrine into the granules was inhibited in a dose-related manner. At 0.7 mM halothane, epinephrine uptake was reduced to 56 per cent of control. The inhibition was completely reversible after removal of halothane from the medium. The inhibitory effect of halothane was not affected by high-osmolar medium. Catecholamine release from isolated chromaffin granules was enhanced by halothane at concentrations of more than 1.3 mM, but was not affected when the concentration was 0.7 mM or less. The results suggest the possiblity that halothane might alter the intracellular storage-turnover process and the subsequent release pattern of catecholamines. The possible mechanisms of this action are discussed.
利用分离出的牛肾上腺髓质嗜铬颗粒，研究了氟烷对嗜铬颗粒中儿茶酚胺释放以及14C-肾上腺素摄取到颗粒中的影响。嗜铬颗粒用微孔滤膜分离，并在含有三磷酸腺苷（ATP）-Mg++的等渗培养基中孵育10分钟。当氟烷浓度达到0.4 mM或更高时，14C-肾上腺素摄取到颗粒中的过程受到剂量相关的抑制。在0.7 mM氟烷时，肾上腺素摄取量降至对照的56%。从培养基中去除氟烷后，这种抑制作用完全可逆。氟烷的抑制作用不受高渗培养基的影响。当氟烷浓度超过1.3 mM时，分离出的嗜铬颗粒中儿茶酚胺的释放增强，但当浓度为0.7 mM或更低时则不受影响。结果提示氟烷可能改变儿茶酚胺的细胞内储存-周转过程及随后的释放模式。本文讨论了这种作用的可能机制。
