Chien M M, Rosazza J P
Appl Environ Microbiol. 1980 Oct;40(4):741-5. doi: 10.1128/aem.40.4.741-745.1980.
Aromatic hydroxylation of the antitumor alkaloid ellipticine by Aspergillus alliaceus results in the formation of both 8- and 9-hydroxyellipticines. Experiments designed to increase yields of microbial hydroxylation focused on enhancing the water solubility of ellipticine in aqueous fermentation media by use of wetting agents or solubilizing polymers, or both. Yields of hydroxylated metabolites were determined by high-performance liquid chromatography with the use of a mu-Bondapak-phenyl column and a mobile phase of acetonitrile--0.1% (NH4)2CO3 (1:1). Polyvinylpyrrolidone (molecular weight, 40,000) and polyoxyethylated vegetable oil (Emulphor 620) increased the water solubility of ellipticine and the yield of hydroxylated metabolites.
蒜曲霉对抗肿瘤生物碱玫瑰树碱的芳族羟基化作用会生成8-羟基玫瑰树碱和9-羟基玫瑰树碱。旨在提高微生物羟基化产量的实验着重于通过使用湿润剂或增溶聚合物或两者兼用来提高玫瑰树碱在水发酵培养基中的水溶性。羟基化代谢物的产量通过高效液相色谱法测定，使用μ-Bondapak-苯基柱和乙腈–0.1% (NH4)2CO3 (1:1)的流动相。聚乙烯吡咯烷酮（分子量40,000）和聚氧乙烯化植物油（乳化剂620）提高了玫瑰树碱的水溶性以及羟基化代谢物的产量。
