Gerritsen M E, Printz M P
Artery. 1980;8(1):56-62.
Microsomes prepared from the brachial artery and the thoracic aorta of atherosclerosis resistant (AR) Show Racer and atherosclerosis prone (AS) White Carneau pigeons were incubated with 14C-prostaglandin endoperoxide (PGH) and prostaglandin products analyzed. The microsomes demonstrated minimal prostacyclin (PGI2) synthetase activity; 6-keto-PGF1 alpha (the hydrolytic breakdown product of PGI2) accounting for less than 2% of total products. Reduced glutathione enhanced PGE2 formation in both the AR and AS preparations identifying an active PGH-PGE isomerase. The AR preparations demonstrated increased PGH-PGE formation with age, reaching maximal activity at 8-9 months, then decreasing at 14 months. The AS group also demonstrated a similar pattern of enzyme activity. These studies indicate that a) unlike the mammalian preparations prostacyclin synthetase does not appear to be a major enzymatic activity of the pigeon aorta, rather, b) PGH-PGE isomerase is the major endoperoxide metabolizing activity in the pigeon aorta and thus, c) a deficiency of prostacyclin production is not involved in the geneis of atherosclerosis in the pigeon.
从抗动脉粥样硬化（AR）赛鸽和易患动脉粥样硬化（AS）白卡诺鸽的肱动脉和胸主动脉制备的微粒体，与14C - 前列腺素内过氧化物（PGH）一起孵育，并对前列腺素产物进行分析。微粒体显示出最小的前列环素（PGI2）合成酶活性；6 - 酮 - PGF1α（PGI2的水解分解产物）占总产物的不到2%。还原型谷胱甘肽增强了AR和AS制剂中PGE2的形成，从而确定了一种活性PGH - PGE异构酶。AR制剂中PGH - PGE的形成随年龄增加，在8 - 9个月时达到最大活性，然后在14个月时下降。AS组也表现出类似的酶活性模式。这些研究表明：a）与哺乳动物制剂不同，前列环素合成酶似乎不是鸽主动脉的主要酶活性，相反，b）PGH - PGE异构酶是鸽主动脉中主要的内过氧化物代谢活性，因此，c）前列环素产生的缺乏与鸽动脉粥样硬化的发生无关。
