Sheehe D M, Green J B, Green M H
Atherosclerosis. 1980 Oct;37(2):301-10. doi: 10.1016/0021-9150(80)90016-7.
Flux (mumole/h) of triglycerides, phospholipids and cholesterol into the thoracic duct lymph was measured in rats receiving a constant intraduodenal infusion of a cholesterol-free oil rich in either polyunsaturated (P/S = 4.8) or saturated (P/S = 0.2) fatty acids. Rats had ad libitum access to a fat-free semi-synthetic diet throughout the experiment. Oils were infused at a rate equivalent to a 10% (w/w) fat-containing diet for at least 18 h prior to collection of lymph; both oils were compared in each animal. Although absorption of the infused oils approximated 100%, triglyceride flux was significantly lower during infusion of the saturated compared to the polyunsaturated oil. Phospholipid and total cholesterol fluxes were not significantly affected by the type of oil, but the percent of lymph total cholesterol which was esterified was slightly but significantly lower during infusion of the unsaturated oil. Using the molar phospholipid/triglyceride ratio as a index of lymph lipoprotein size, it was found that absorption of the oil rich in polyunsaturated fatty acids resulted in an increase in the mean size of lymph lipoproteins. The potential significance of an influence of dietary fat saturation on lymph lipoprotein size and cholesterol esterification for the ultimate metabolic fate of absorptive lipoprotein constituents is discussed.
在持续十二指肠内输注富含多不饱和脂肪酸（P/S = 4.8）或饱和脂肪酸（P/S = 0.2）的无胆固醇油的大鼠中，测量了甘油三酯、磷脂和胆固醇进入胸导管淋巴的通量（微摩尔/小时）。在整个实验过程中，大鼠可随意获取无脂肪的半合成饮食。在收集淋巴前至少18小时，以相当于含10%（w/w）脂肪饮食的速率输注油；在每只动物中对两种油进行比较。尽管输注油的吸收率接近100%，但与多不饱和油相比，在输注饱和油期间甘油三酯通量显著降低。磷脂和总胆固醇通量不受油类型的显著影响，但在输注不饱和油期间，淋巴总胆固醇中被酯化的百分比略有但显著降低。以摩尔磷脂/甘油三酯比值作为淋巴脂蛋白大小的指标，发现富含多不饱和脂肪酸的油的吸收导致淋巴脂蛋白平均大小增加。讨论了饮食脂肪饱和度对淋巴脂蛋白大小和胆固醇酯化的影响对于吸收性脂蛋白成分最终代谢命运的潜在意义。
