Edwards R G, Steptoe P C, Fowler R E, Baillie J
Br J Obstet Gynaecol. 1980 Sep;87(9):769-79. doi: 10.1111/j.1471-0528.1980.tb04612.x.
During laparoscopy for the collection of preovulatory oocytes the ovaries were inspected and the numbers of Graafian follicles were counted. Most patients had one large preovulatory follicle but three patients had two and might have had twin ovulations. The large follicle was in the left ovary in 9 patients and in the right ovary in 11. It could be aspirated easily in most patients, viscous follicular fluid, presumably rich in hyaluronic acid, appeared to accumulate in preovulatory follicles between 18 and 27 hours after the luteinizing hormone surge. The content of steroids in follicular fluids indicated that the largest follicle was preovulatory in most patients, the smaller follicles being non-ovulatory. Granulosa cells aspirated from the large preovulatory follicles were active on the delta 4 pathway and able to aromatise androgens to oestrogens, but did not undertake conversions on the delta 5 pathway.
在腹腔镜采集排卵前卵母细胞的过程中，对卵巢进行了检查并计数了格拉夫卵泡的数量。大多数患者有一个大的排卵前卵泡，但有三名患者有两个，可能发生了双排卵。9名患者的大卵泡在左侧卵巢，11名患者的在右侧卵巢。在大多数患者中，卵泡液很容易被吸出，推测富含透明质酸的粘性卵泡液似乎在促黄体生成素激增后18至27小时在排卵前卵泡中积聚。卵泡液中的类固醇含量表明，大多数患者中最大的卵泡是排卵前的，较小的卵泡不排卵。从大的排卵前卵泡中吸出的颗粒细胞在δ4途径上活跃，能够将雄激素芳香化为雌激素，但不进行δ5途径的转化。
