Channing C P, Coudert S P
Endocrinology. 1976 Mar;98(3):590-7. doi: 10.1210/endo-98-3-590.
In order to ascertain which ovarian cell type within the follicle is the source of preovulatory estrogen secretion in vivo, ovarian venous, as well as peripheral venous, blood was collected prior to, 5 min, 30 min, and 120 min after the removal of follicular fluid and granulosa cells from 17 monkeys. In addition, estrogen, progesterone, and progestins were measured in the peripheral blood, ovarian venous blood, and follicular fluid of the follicle-containing and contralateral ovary in 24 monkeys, in order to prove that the preovulatory follicle is the principal source of estrogen. Estradiol was the principal estrogen and was secreted in larger amounts by the ovary with the large preovulatory follicle (7-10 mm in diameter) compared with the contralateral ovary. In 15 experiments ovarian venous estrogen (3934 +/- 798 pg/ml, mean +/- SE) in the vein draining the large follicle-containing ovary was usually 5-15-fold higher than peripheral plasma estrogen levels which were 307 +/- 31 pg/ml. The contralateral ovary secreted a small amount of estrogen (654 +/- 162 pg/ml). Follicular fluid contained large amounts of estrogen (2754 +/- 695 ng/ml) with levels which did not always correlate well with peripheral plasma or ovarian venous estrogen. Ovaries containing non-preovulatory or recently ovulated follicles secreted less estrogen. The removal of granulosa cells and follicular fluid from the preovulatory follicle led to no significant decrease (P greater than 0.5) in ovarian venous secretion of estrogen after a 5, 30, or 120 min time interval. This would indicate that, within the time constraints of this experiments, the follicular fluid and granulosa cells contribute relatively little to ovarian venous estrogen and that thecal cells of the large preovulatory follicle alone can secrete more of the estrogen into the ovarian vein.
为了确定卵泡内哪种卵巢细胞类型是体内排卵前雌激素分泌的来源，在从17只猴子身上移除卵泡液和颗粒细胞之前、移除后5分钟、30分钟和120分钟，采集了卵巢静脉血以及外周静脉血。此外，为了证明排卵前卵泡是雌激素的主要来源，还对24只猴子含卵泡侧卵巢和对侧卵巢的外周血、卵巢静脉血和卵泡液中的雌激素、孕酮和孕激素进行了测量。雌二醇是主要的雌激素，与对侧卵巢相比，有大的排卵前卵泡（直径7 - 10毫米）的卵巢分泌的雌二醇量更多。在15次实验中，引流含大卵泡卵巢的静脉中的卵巢静脉雌激素（3934±798皮克/毫升，平均值±标准误）通常比外周血浆雌激素水平高5 - 15倍，外周血浆雌激素水平为307±31皮克/毫升。对侧卵巢分泌少量雌激素（654±162皮克/毫升）。卵泡液中含有大量雌激素（2754±695纳克/毫升），其水平与外周血浆或卵巢静脉雌激素水平并不总是密切相关。含有未排卵或刚排卵卵泡的卵巢分泌的雌激素较少。在5分钟、30分钟或120分钟的时间间隔后，从排卵前卵泡中移除颗粒细胞和卵泡液，卵巢静脉雌激素分泌没有显著下降（P大于0.5）。这表明，在本实验的时间限制内，卵泡液和颗粒细胞对卵巢静脉雌激素的贡献相对较小，仅大的排卵前卵泡的膜细胞就能将更多的雌激素分泌到卵巢静脉中。
