Jennings H J, Lugowski C, Young N M
Biochemistry. 1980 Sep 30;19(20):4712-9. doi: 10.1021/bi00561a026.
The complex cell-wall polysaccharide, C-substance, was isolated from Streptococcus pneumoniae type 1 and purified by DEAE-cellulose (HCO3(-) form) and Sephadex column chromatography. The complete structure of this antigen was obtained by the application of methylation and 1H NMR and 13C NMR spectroscopic techniques to a series of oligosaccharide fragments obtained by the selective degradation of the N-acetylated antigen. Native C-substance is composed of the following repeating unit: beta-D-Glup-1 leads to 3-alpha-AAT-Galp-1 leads to 4-alpha-D-GalNAcp-1 leads to 3-beta-D-GalNH2p-1 leads to 1'-ribitol-5-phosphate where AATGal is 2-acetamido-4-amino-2,4,6-trideoxygalactose. Phosphocholine substituents are situated at O(6) of the unacetylated galactosamine residues, and the repeating units are linked through a diphosphate ester from ribitol to O(6) of the beta-D-glucopyranose residue. This structure has also been shown to be common to C-substances prepared from a number of other pneumococcal types based on the criterion of their identical 13C NMR spectra.
从1型肺炎链球菌中分离出复杂的细胞壁多糖C物质，并通过DEAE - 纤维素（HCO3(-) 形式）和葡聚糖凝胶柱色谱法进行纯化。通过对一系列通过N - 乙酰化抗原的选择性降解获得的寡糖片段应用甲基化、1H NMR和13C NMR光谱技术，得到了该抗原的完整结构。天然C物质由以下重复单元组成：β - D - 葡糖 - 1→3 - α - AAT - 半乳糖 - 1→4 - α - D - 氨基半乳糖 - 1→3 - β - D - 半乳糖胺 - 1→1'-核糖醇 - 5 - 磷酸，其中AATGal是2 - 乙酰氨基 - 4 - 氨基 - 2,4,6 - 三脱氧半乳糖。磷胆碱取代基位于未乙酰化的半乳糖胺残基的O(6) 位，重复单元通过从核糖醇到β - D - 吡喃葡萄糖残基的O(6) 的二磷酸酯连接。基于它们相同的13C NMR光谱标准，该结构也已被证明对于从许多其他肺炎球菌类型制备的C物质是共同的。
