Böddi B, Soós J, Láng F
Biochim Biophys Acta. 1980 Nov 5;593(1):158-65. doi: 10.1016/0005-2728(80)90017-1.
Spectral properties of protochlorophyll ()PChl) forms were investigated in solid-film model systems by absorption fluorescence and circular dichroism (CD) spectroscopy. The solid films were prepared from diethyl ether solution of PChl on a cover glass surface by evaporation of the solvent. After preparation the films usually showed an absoprtion maximum at 635 nm or in some cases at 640 nm. The PChl form with 635 nm absorption maximum had no CD signal, whilst the films with absorption maximum at 640 nm gave an intense negative CD band at about 640 nm and a positive one at 668 nm. The treatment of the films with ammonia or acetone vapour resulted in a red shift of the absorption maximum from 635 nm or 640 nm to 650 nm. The study of the CD spectra of the films with different PChl forms showed that, depending on the treatment, forms of PChl with similar absorption and fluorescence spectra, but with opposite CD signals, can exist. It is suggested that the differences of the CD spectra are mainly due to different arrangements of the aggregates.
通过吸收荧光光谱和圆二色性（CD）光谱，在固体薄膜模型系统中研究了原叶绿素（PChl）形式的光谱特性。固体薄膜是通过在盖玻片表面蒸发PChl的乙醚溶液制备而成。制备后，薄膜通常在635nm处有一个吸收最大值，在某些情况下在640nm处。最大吸收波长为635nm的PChl形式没有CD信号，而最大吸收波长在640nm的薄膜在约640nm处有一个强烈的负CD带，在668nm处有一个正CD带。用氨或丙酮蒸汽处理薄膜会导致吸收最大值从635nm或640nm红移到650nm。对具有不同PChl形式的薄膜的CD光谱研究表明，根据处理方式的不同，可能存在吸收和荧光光谱相似但CD信号相反的PChl形式。研究表明，CD光谱的差异主要是由于聚集体的排列不同。
