来自北美独行菜的水溶性叶绿素蛋白的结晶
Crystallization of water-soluble chlorophyll-proteins from Lepidium virginicum.
作者信息
Murata T, Itoh R, Yakushiji E
出版信息
Biochim Biophys Acta. 1980 Nov 5;593(1):167-70. doi: 10.1016/0005-2728(80)90018-3.
PMID:7426644
Abstract
Water-soluble chlorophyll-proteins were prepared from leaves of Lepidium virginicum, by means of ammonium sulfate fractionation followed by column chromatography on DEAE-cellulose and Sephacryl S-200. After intensive purification the chlorophyll-proteins were crystallized by dialysis against an ammonium sulfate solution.
摘要
水溶性叶绿素蛋白是从北美独行菜叶片中制备的，方法是先通过硫酸铵分级分离，然后在DEAE-纤维素和Sephacryl S-200上进行柱色谱分离。经过深度纯化后，通过对硫酸铵溶液进行透析使叶绿素蛋白结晶。
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