Murata T, Ishikawa C
Biochim Biophys Acta. 1981 Apr 13;635(2):341-7. doi: 10.1016/0005-2728(81)90032-3.
Two kinds of water-soluble chlorophyll-protein complexes were prepared from leaves of Lepidium virginicum L., one (CP661) from the plant cultivated in a green house from seeds collected near Mono Lake, CA, and the other (CP-663) from a plant collected at Narashino, Chiba, Japan, by ammonium sulfate fractionation followed by column chromatography on DEAE-cellulose and Sephacryl S-200. The chlorophyll . proteins were further purified by crystallization. CP661 has absorption peaks at 661, 468, 439, 419, 380, 339 and 272 nm. CP663 had absorption peaks at 663, 469, 438, 419, 379, 338 and 272 nm. Estimated molecular weights were 78 000 for CP661 and 80 000 for CP663 by gel filtration chromatography and 83 000 for CP661 and 107 000 for CP663 by an equilibrium sedimentation method. 1 mol chlorophyll . protein contained 4 mol chlorophyll a and b with ratios of 1.0 in CP661 and 1.6 to 1.9 in CP663, but no carotenoids. These characters are different from those of chlorophyll-protein complexes which are prepared from the thylakoid membranes of chloroplasts with detergents.
从北美独行菜叶片中制备了两种水溶性叶绿素 - 蛋白质复合物，一种（CP661）来自于用从加利福尼亚州莫诺湖附近采集的种子在温室中培育的植株，另一种（CP - 663）来自于在日本千叶县习志野采集的植株，制备方法是先进行硫酸铵分级分离，然后在DEAE - 纤维素和Sephacryl S - 200上进行柱色谱。通过结晶进一步纯化叶绿素 - 蛋白质。CP661在661、468、439、419、380、339和272 nm处有吸收峰。CP663在663、469、438、419、379、338和272 nm处有吸收峰。通过凝胶过滤色谱法估计CP661的分子量为78000，CP663为80000；通过平衡沉降法估计CP661为83000，CP663为107000。1摩尔叶绿素 - 蛋白质含有4摩尔叶绿素a和b，在CP661中比例为1.0，在CP663中为1.6至1.9，但不含类胡萝卜素。这些特性与用去污剂从叶绿体类囊体膜制备的叶绿素 - 蛋白质复合物不同。
