Olenev S N, Shkliaeva L D
Arkh Anat Gistol Embriol. 1978 Dec;75(12):59-65.
In order to reveal dehydrogenase and diaphorase in spinal ganglia neurons of 12-day-old chick embryos in cryostat sections, the following modifications of the medium were used: for dehydrogenase - sodium salt substrate 50 mM, NAD or NADPh 0.75 mM, nitro-BT 0.61 mM, phosphate buffer pH 7.2 15 mM, NaCl 50 mM, MgCL2 5 mM, for diaphorase - NAD-N2 or NADHh-N2 0.78-0.66 mM, NaCl 100 mM. To compare relative activity of the enzymes (optic density of histochemical preparations determined cytophotometrically) it is suggested to calculate the values obtained during proportional development of the staining regarding the time unit (hour). The possibility to compare the data obtained with the results of biochemical investigations is discussed, as well as an attempt is made to represent graphically metabolic peculiarities of various cell types.
为了在低温切片中揭示12日龄鸡胚脊髓神经节神经元中的脱氢酶和黄递酶，对培养基进行了以下修改：对于脱氢酶——50 mM的钠盐底物、0.75 mM的NAD或NADPh、0.61 mM的硝基蓝四氮唑、15 mM pH 7.2的磷酸盐缓冲液、50 mM的NaCl、5 mM的MgCl2；对于黄递酶——0.78 - 0.66 mM的NAD - N2或NADH - N2、100 mM的NaCl。为了比较酶的相对活性（通过细胞光度法测定组织化学制剂的光密度），建议计算在染色比例发展过程中以时间单位（小时）获得的值。讨论了将获得的数据与生化研究结果进行比较的可能性，并尝试以图形方式展示各种细胞类型的代谢特性。
