Olenev S N, Shkliaeva L D
Arkh Anat Gistol Embriol. 1979 Apr;76(4):59-64.
Enzymatic activity was investigated in spinal ganglia of 10-day-old chick embryo and in 10-day-old cultures of these ganglia (the method of "flying glasses"). Histochemical preparations were photometrically treated with the one-wave plug-method in the device MCFB-1 (LOMO) with the diameter of the probe 8 mkm. As the result of the investigations on optic density, a definite conclusion was made about relative activity of 15 enzymes and a general scheme of neuronal metabolism was graphically presented under the conditions of cultivation, enzymatic activity of krebs cycle was demonstrated to decrease, while that of diaphorase and lactate dehydrogenase to increase. The data on topography of enzymatic distribution in neurons, satellites, lemmoncytes and fibroblasts were also presented.
在10日龄鸡胚的脊髓神经节以及这些神经节的10日龄培养物（“飞片法”）中研究了酶活性。组织化学制剂在MCFB - 1（LOMO）装置中采用单波插塞法进行光度处理，探头直径为8微米。通过对光密度的研究，得出了关于15种酶相对活性的明确结论，并以图表形式展示了培养条件下神经元代谢的总体方案，结果表明三羧酸循环的酶活性降低，而黄递酶和乳酸脱氢酶的活性增加。还给出了神经元、卫星细胞、施万细胞和成纤维细胞中酶分布的拓扑学数据。
