Welsh M J, Widdicombe J H, Nadel J A
J Appl Physiol Respir Environ Exerc Physiol. 1980 Nov;49(5):905-9. doi: 10.1152/jappl.1980.49.5.905.
We measured the rate of fluid movement across the tracheal epithelium of the dog by an electrical method, modified from that originally described by Wiedner (Rev. Sci. Instrum. 47: 775-6, 1976). The base-line volume flow (Jv) from the submucosa to the mucosa was 44 +/- 23 nl.min-1.cm-2 (mean +/- SE; n = 6). In only three of the tissues was the resting Jv significantly greater than zero. Stimulation with 2 X 10(-3) M aminophylline (a drug that we have shown stimulates Cl secretion under short-circuit conditions) increased Jv in all tissues to a mean of 137 +/- 29 nl.min-1.cm-2 (P < 0.001). We also measured the unidirectional fluxes of Cl with the tissue at its spontaneous electrical potential. During the base-line period, the net flux of Cl was not significantly different from zero. During stimulation with aminophylline, Cl secretion increased to a mean of 1.44 +/- 0.50 microneq.cm-2.h-1. Comparison of the stimulated values of Jv and net Cl secretion suggests that fluid secretion by the trachea is isotonic during stimulation with aminophylline.
我们采用一种电学方法测量了犬气管上皮的液体转运速率，该方法是对维德纳最初描述的方法（《科学仪器评论》47: 775 - 6, 1976）进行了改进。从黏膜下层到黏膜层的基线体积流量（Jv）为44 ± 23 nl·min⁻¹·cm⁻²（平均值 ± 标准误；n = 6）。在这些组织中，只有三个组织的静息Jv显著大于零。用2×10⁻³ M氨茶碱（我们已证明在短路条件下该药物能刺激氯离子分泌）刺激后，所有组织中的Jv均增加到平均值137 ± 29 nl·min⁻¹·cm⁻²（P < 0.001）。我们还在组织处于自发电位时测量了氯离子的单向通量。在基线期，氯离子的净通量与零无显著差异。在用氨茶碱刺激期间，氯离子分泌增加到平均值1.44 ± 0.50微当量·cm⁻²·h⁻¹。对刺激后的Jv值和氯离子净分泌的比较表明，在用氨茶碱刺激期间，气管的液体分泌是等渗的。
