血管活性肠肽对犬气管上皮离子转运的影响。

Effect of vasoactive intestinal peptide on ion transport across dog tracheal epithelium.

作者信息

Nathanson I, Widdicombe J H, Barnes P J

出版信息

J Appl Physiol Respir Environ Exerc Physiol. 1983 Dec;55(6):1844-8. doi: 10.1152/jappl.1983.55.6.1844.

DOI:10.1152/jappl.1983.55.6.1844

Abstract

Under short-circuit conditions, vasoactive intestinal peptide (VIP) did not alter net Na+ movement but selectively stimulated net Cl- secretion across dog tracheal epithelium with a high affinity (Km congruent to 10(-8) M). The increase in Cl- secretion was not different from the rise in short-circuit current (Isc). However, stimulation of Cl- secretion was not maximal, because the addition of isoproterenol (10(-6) M) to VIP-treated tissues further increased the Isc by 54%. The effect of exogenous VIP was not blocked by a combination of atropine, phentolamine, propranolol (10(-5) or 10(-6) M), or tetrodotoxin (10(-6) M). Under open-circuit conditions, VIP caused an increase in the net secretion of Cl- and Na+, but the changes did not reach statistical significance. We conclude that VIP acts directly on receptors on the surface of epithelial cells to stimulate active Cl- secretion. The abundance of VIP nerves in the submucosa suggests that VIP may be important in regulation of fluid movement across the epithelium.

摘要

在短路条件下，血管活性肠肽（VIP）不会改变净Na⁺转运，但能以高亲和力（Km约为10⁻⁸M）选择性刺激犬气管上皮细胞的净Cl⁻分泌。Cl⁻分泌的增加与短路电流（Isc）的升高无差异。然而，Cl⁻分泌的刺激并非最大程度，因为向经VIP处理的组织中添加异丙肾上腺素（10⁻⁶M）可使Isc进一步增加54%。外源性VIP的作用不会被阿托品、酚妥拉明、普萘洛尔（10⁻⁵或10⁻⁶M）或河豚毒素（10⁻⁶M）的组合所阻断。在开路条件下，VIP导致Cl⁻和Na⁺的净分泌增加，但这些变化未达到统计学显著水平。我们得出结论，VIP直接作用于上皮细胞表面的受体以刺激主动Cl⁻分泌。黏膜下层中丰富的VIP神经表明，VIP可能在调节上皮细胞间的液体转运中起重要作用。