高效液相色谱法测定糖蜜中的果糖、葡萄糖和蔗糖

High performance liquid chromatographic determination of fructose, glucose, and sucrose in molasses.

作者信息

Damon C E, Pettitt B C

出版信息

J Assoc Off Anal Chem. 1980 May;63(3):476-80.

Abstract

Fructose, glucose, and sucrose were determined quantitatively in molasses by high performance liquid chromatography (HPLC) using maltose as an internal standard. After a simple cleanup procedure, chromatographic separation required less than 20 min for the 17 molasses samples. HPLC gave lower results for reducing sugars (fructose plus glucose) and for sucrose by an average of 3.9 and 1.2%, respectively, when compared to results obtained by classical methods. The lower values are believed to result from elimination of interfering substances by this method. The values probably reflect more accurately the true composition of the molasses. Repetitive injections of a standard solution indicated a coefficient of variation of 2.0% for fructose and glucose and of 2.2% for sucrose.

摘要

采用高效液相色谱法（HPLC），以麦芽糖作为内标，对糖蜜中的果糖、葡萄糖和蔗糖进行了定量测定。经过简单的净化程序后，17个糖蜜样品的色谱分离时间不到20分钟。与经典方法得到的结果相比，HPLC测定的还原糖（果糖加葡萄糖）和蔗糖结果分别平均低3.9%和1.2%。据信，较低的值是由于该方法消除了干扰物质所致。这些值可能更准确地反映了糖蜜的真实成分。重复进样标准溶液表明，果糖和葡萄糖的变异系数为2.0%，蔗糖的变异系数为2.2%。