Singhawangcha S, Poole C F, Zlatkis A
J Chromatogr. 1980 Oct 10;183(4):433-9.
Guaifenesin after extraction from plasma with an organic solvent can be selectively derivatized with 2,4-dichlorobenzeneboronic acid and determined by gas chromatography with electron-capture detection. The detection limit for guaifenesin was 15 ng ml-1 for a 2.0-ml plasma sample. The mass spectra of the boronate derivatives of guaifenesin and mephenesin, used as internal standard, show good molecular ions with characteristic modes of fragmentation useful for their identification.
用有机溶剂从血浆中提取愈创甘油醚后，它可与2,4 - 二氯苯硼酸进行选择性衍生化反应，并通过带有电子捕获检测的气相色谱法进行测定。对于2.0毫升血浆样品，愈创甘油醚的检测限为15纳克/毫升。用作内标的愈创甘油醚和美芬新的硼酸酯衍生物的质谱显示出良好的分子离子峰以及有助于其鉴定的特征性裂解模式。
