Loomis C W, Brien J F
J Chromatogr. 1981 Mar 13;222(3):421-8.
A sensitive and selective method for the measurement of carbimide, the hydrolytic product of calcium carbimide, in plasma is described. The procedure involves extraction with ethyl acetate, derivatization with heptafluorobutyric anhydride and analysis by gas-liquid chromatography with electron-capture detection. The lower limit of sensitivity of the assay is 5.0 ng/ml carbimide in plasma. The overall accuracy of the procedure is 96.1% with a coefficient of variation not exceeding 8.7%. This assay has been used to investigate the time-course of plasma carbimide concentration in the rat following oral administration of calcium carbimide.
本文描述了一种灵敏且具选择性的方法，用于测定血浆中卡巴咪（卡巴咪钙的水解产物）。该方法包括用乙酸乙酯萃取、用七氟丁酸酐衍生化，以及通过带有电子捕获检测的气液色谱法进行分析。该测定法的灵敏度下限为血浆中5.0纳克/毫升的卡巴咪。该方法的总体准确度为96.1%，变异系数不超过8.7%。此测定法已用于研究大鼠口服卡巴咪钙后血浆中卡巴咪浓度的时间进程。
