Stafford H A, Fanger M W
J Immunol. 1980 Dec;125(6):2461-6.
The distribution of IgA binding lymphoid cells in the rabbit was determined by using ox erythrocytes coated with specific secretory IgA. After overnight incubation at 37 degrees C, the average percentage of IgA binding lymphocytes detected in major IgA-producing organs (Peyer's patches, 14.4%; appendix, 8.4%; and mesenteric lymph nodes, 11.3%), was generally higher than that detected in systemic lymphoid tissue. Lymphocytes bearing receptors for IgA (RFc alpha) were detected immediately after isolation only in the spleen and failed to appear after overnight incubation only in the thymus. That these receptors were distinct from receptors for IgM or IgG was demonstrated by cross-blocking studies with purified IgG, IgM, and IgA immunoglobulins. RFc alpha were sensitive to pronase digestion at 1 mg/ml and were regenerated with overnight incubation. In preliminary studies designed to evaluate modulation of this receptor, it was found that a significant increase in the percentage of cells expressing RFc alpha could be induced by antibodies specific for rabbit immunoglobulin. This information as a whole is consistent with the premise that RFc alpha-expressing lymphocytes may be involved in the regulation or expression of IgA-mediated immunity.
通过使用包被有特异性分泌型IgA的牛红细胞来测定兔体内IgA结合淋巴细胞的分布。在37℃过夜孵育后，在主要的IgA产生器官（派氏集合淋巴结，14.4%；阑尾，8.4%；肠系膜淋巴结，11.3%）中检测到的IgA结合淋巴细胞的平均百分比通常高于在全身淋巴组织中检测到的百分比。仅在脾脏中分离后立即检测到带有IgA受体（RFcα）的淋巴细胞，而仅在胸腺中过夜孵育后未出现。用纯化的IgG、IgM和IgA免疫球蛋白进行的交叉阻断研究表明，这些受体与IgM或IgG的受体不同。RFcα对1mg/ml的链霉蛋白酶消化敏感，并通过过夜孵育得以再生。在旨在评估该受体调节作用的初步研究中，发现兔免疫球蛋白特异性抗体可诱导表达RFcα的细胞百分比显著增加。总体而言，这些信息与表达RFcα的淋巴细胞可能参与IgA介导的免疫调节或表达这一前提一致。
