Weiner R S, Shah V O
J Immunol Methods. 1980;36(2):89-97. doi: 10.1016/0022-1759(80)90034-4.
Monocytes from human peripheral blood were purified by elutriation centrifugation. Up to 1.5 x 10(9) peripheral blood mononuclear cells could be separated in isotonic media to yield 6--8 x 10(8) lymphocytes with 95% purity and 1.5--2.5 x 10(8) monocytes with greater than 90% purity. The temperature at which elutriation was performed determined the purity of the monocyte fraction. Both the lymphocyte and monocyte fractions were characterized by cell sizing and histochemical staining. Most of the myeloperoxidase positive mononuclear cells (monocytes) had a modal volume of 378 micrometer 3 while 10% were within the modal volume of the lymphocytes, 131 micrometer 3. Thus, there are two populations of cells with histochemical properties of monocytes which are separable by size. The collection and isolation of human peripheral blood monocyte populations in large numbers will facilitate studies of their functional characteristics.
通过淘洗离心法纯化人外周血中的单核细胞。在等渗培养基中，可分离出多达1.5×10⁹个外周血单核细胞，得到纯度为95%的6 - 8×10⁸个淋巴细胞和纯度大于90%的1.5 - 2.5×10⁸个单核细胞。进行淘洗的温度决定了单核细胞组分的纯度。淋巴细胞和单核细胞组分均通过细胞大小测定和组织化学染色进行表征。大多数髓过氧化物酶阳性单核细胞（单核细胞）的模态体积为378立方微米，而10%的细胞处于淋巴细胞的模态体积范围内，即131立方微米。因此，存在两类具有单核细胞组织化学特性的细胞群体，可通过大小进行分离。大量收集和分离人外周血单核细胞群体将有助于对其功能特性的研究。
