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Isolation of human monocytes by rosetting with antibody coated human erythrocytes and isopycnic gradient centrifugation.通过与抗体包被的人红细胞进行玫瑰花结形成及等密度梯度离心法分离人单核细胞。
Clin Exp Immunol. 1982 Jul;49(1):225-9.
2
Purified canine monocytes fail to confer concanavalin-A responsiveness to accessory-cell-depleted lymphocytes.纯化的犬单核细胞无法赋予对伴细胞耗竭淋巴细胞的刀豆球蛋白A反应性。
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Enumeration and isolation of human T and B lymphocytes by rosette formation with antibody-coated erythrocytes.通过与抗体包被红细胞形成玫瑰花结来计数和分离人T淋巴细胞和B淋巴细胞。
Clin Exp Immunol. 1975 Oct;22(1):62-71.
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Immunoglobulin-positive mononuclear cells in human peripheral blood: detection by mixed anti-globulin and direct anti-globulin-rosetting reactions.人外周血中免疫球蛋白阳性单核细胞：通过混合抗球蛋白和直接抗球蛋白玫瑰花结反应进行检测
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Enumeration and isolation of rabbit T and B lymphocytes by using antibody-coated erythrocytes.利用抗体包被的红细胞对兔T淋巴细胞和B淋巴细胞进行计数与分离。
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Mitogenic responsiveness and monocyte-lymphocyte interaction of early and late rosette-forming cell populations of human peripheral blood lymphocytes.人外周血淋巴细胞早期和晚期玫瑰花结形成细胞群体的促有丝分裂反应性及单核细胞-淋巴细胞相互作用
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Canine lymphocyte subpopulations.犬淋巴细胞亚群
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A new density gradient for the separation of large quantities of rosette-positive and rosette-negative cells.一种用于分离大量玫瑰花结阳性和玫瑰花结阴性细胞的新型密度梯度。
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2
Monocyte subsets in the production of inhibitory factor by Candida albicans-activated human T cells.白色念珠菌激活的人T细胞产生抑制因子过程中的单核细胞亚群
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3
The altered expression of MHC-class II determinants on monocytes of cancer patients.癌症患者单核细胞上MHC-II类决定簇的表达改变。
Cancer Immunol Immunother. 1988;27(2):171-6. doi: 10.1007/BF00200023.

本文引用的文献

1
Activation of human monocytes for nitroblue tetrazolium reduction and the suppression of lymphocyte response to mitogens.人单核细胞对硝基蓝四氮唑还原的激活作用以及淋巴细胞对有丝分裂原反应的抑制作用。
Clin Exp Immunol. 1980 Aug;41(2):309-16.
2
Inhibitory factor(s) of lymphoproliferation produced by synovial fluid mononuclear cells from rheumatoid arthritis patients: the role of monocytes in suppression.类风湿关节炎患者滑液单核细胞产生的淋巴细胞增殖抑制因子：单核细胞在抑制中的作用
J Immunol. 1980 Sep;125(3):1087-92.
3
Generation and specificity of a heteroantiserum to human peripheral blood monocytes.人外周血单核细胞异种抗血清的产生及特异性
Clin Immunol Immunopathol. 1980 Aug;16(4):438-46. doi: 10.1016/0090-1229(80)90185-3.
4
Monocyte contamination in Ficoll-Hypaque mononuclear cell concentrates.Ficoll-泛影葡胺单核细胞浓缩液中的单核细胞污染
J Immunol Methods. 1980;32(3):215-21. doi: 10.1016/0022-1759(80)90187-8.
5
An extension of the 51Cr-release assay for the estimation of mouse cytotoxins.用于估计小鼠细胞毒素的51铬释放试验的扩展。
Transplantation. 1968 Sep;6(6):761-4. doi: 10.1097/00007890-196809000-00002.
6
The IgG receptor: an immunological marker for the characterization of mononuclear cells.免疫球蛋白G受体：一种用于单核细胞特征鉴定的免疫标志物。
Immunology. 1969 Jul;17(1):7-21.
7
A rapid method for the isolation of functional thymus-derived murine lymphocytes.一种分离功能性胸腺来源的小鼠淋巴细胞的快速方法。
Eur J Immunol. 1973 Oct;3(10):645-9. doi: 10.1002/eji.1830031011.
8
The percentage of monocytes among "mononuclear" cell fractions obtained from normal human blood.从正常人血液中获得的“单核”细胞组分中单核细胞的百分比。
J Immunol. 1974 Jan;112(1):234-40.
9
Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.从人血中分离单核细胞和粒细胞。通过一次离心分离单核细胞，通过离心和1g沉降相结合的方法分离粒细胞。
Scand J Clin Lab Invest Suppl. 1968;97:77-89.
10
Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.人类T和B淋巴细胞的表面标志物。I. 大量与绵羊红细胞形成非免疫性玫瑰花结的淋巴细胞。
J Exp Med. 1972 Aug 1;136(2):207-15. doi: 10.1084/jem.136.2.207.

通过与抗体包被的人红细胞进行玫瑰花结形成及等密度梯度离心法分离人单核细胞。

Isolation of human monocytes by rosetting with antibody coated human erythrocytes and isopycnic gradient centrifugation.

作者信息

Zembala M, Uracz W, Ruggiero I, Lemmel E M

出版信息

Clin Exp Immunol. 1982 Jul;49(1):225-9.

PMID:6982132
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1536639/
Abstract

Human erythrocytes O,Rh+ (D) coated with hyperimmune human anti-serum formed rosettes primarily with monocytes in peripheral blood mononuclear cell suspensions. The rosetting cells (EAhu-FRC), separated on a Lymphoprep gradient were found in the pellet and were up to 95% pure monocytes as judged by cytochemical criteria. A two step procedure involving depletion of T lymphocytes followed by the isolation of EAhu-RFC yielded monocytes up to 98% pure. The isolated cells did not respond to mitogens in vitro, but they act as accessory cells in mitogen-induced T lymphocyte proliferation. This simple method may be useful in studying monocyte function in human disease as it does not require a large amount of blood.

摘要

用人抗血清包被的人O型、Rh+(D)红细胞，主要在外周血单核细胞悬液中与单核细胞形成玫瑰花结。在淋巴细胞分离液梯度上分离得到的玫瑰花结形成细胞（EAhu-FRC）存在于沉淀中，根据细胞化学标准判断，其单核细胞纯度高达95%。采用两步法，先去除T淋巴细胞，再分离EAhu-RFC，可得到纯度高达98%的单核细胞。分离出的细胞在体外对有丝分裂原无反应，但在有丝分裂原诱导的T淋巴细胞增殖中起辅助细胞的作用。这种简单方法可能有助于研究人类疾病中的单核细胞功能，因为它不需要大量血液。