Bagchi N, Brown T R, Shivers B, Mack R E
J Endocrinol. 1980 Sep;86(3):413-8. doi: 10.1677/joe.0.0860413.
The rates of thyroglobulin hydrolysis and iodothyroine release from mouse thyroid glands were studied in vitro. Recently iodinated thyroglobulin ('new pool') had been labelled during life by injection of 131I 3 h before removal of the thyroid, 'old pool' thyroglobulin had been labelled by the administration of 125I in the drinking water for 1 week starting 3 weeks earlier. Chromatogrphic analysis of pronase digests of the thyroid glands showed that the iodothyronine content of the old and new pools were 19.5 and 7.4 per cent respectively. In the basal state the rate of thyroglobulin hydrolysis was lower from the old pool but the rate of hormone secretion was similar from both pools. Thyrotrophin (TSH) increased the rate of thyroglobulin hydrolysis and hormone release from both pools by up to four to six times the basal rate, the effect being maximal 2 h after administration of TSH and lasting for 6-8 h. The rate of thyroglobulin hydrolysis after TSH was similar in both pools but the rate of release of labelled iodothyronines was significantly higher from from the old pool. These studies have indicated that although hydrolysis of thyroglobulin paroceeds faster in the new pool than in the old ('last come, first served' hypothesis) neverrtheless there is no difference in the rate of hormone secretion from the two pools, and hydrolysis in both pools is affected by TSH.
对小鼠甲状腺球蛋白水解速率以及碘甲状腺原氨酸从甲状腺释放的速率进行了体外研究。近期碘化的甲状腺球蛋白（“新池”）在活体中通过在摘除甲状腺前3小时注射131I进行标记，“旧池”甲状腺球蛋白则通过从3周前开始在饮水中给予125I持续1周进行标记。对甲状腺的链霉蛋白酶消化产物进行色谱分析表明，旧池和新池的碘甲状腺原氨酸含量分别为19.5%和7.4%。在基础状态下，旧池的甲状腺球蛋白水解速率较低，但两个池的激素分泌速率相似。促甲状腺激素（TSH）使两个池的甲状腺球蛋白水解速率和激素释放速率提高至基础速率的四到六倍，该效应在给予TSH后2小时达到最大，并持续6 - 8小时。TSH作用后，两个池的甲状腺球蛋白水解速率相似，但旧池标记碘甲状腺原氨酸的释放速率明显更高。这些研究表明，尽管新池中甲状腺球蛋白的水解比旧池更快（“后到先得”假说），但两个池的激素分泌速率并无差异，且两个池的水解均受TSH影响。
