Metzger Z, Buchner A, Gorsky M
J Forensic Sci. 1980 Oct;25(4):742-9.
Gustafson's method for age determination from teeth is based on the evaluation of ground sections of teeth. Six age-associated parameters are evaluated in the ground section and are compared to a regression curve of age versus the age-associated changes. Two of these changes, transparency of radicular dentin and secondary dentin, have the highest correlation with age. The evaluation of these parameters only from thin (0.25-mm) ground sections may lead to an artificially high "secondary dentin value." These artifacts may be caused by the attempt to include the whole pulp chamber and root canal in a 0.25-mm-thick ground section and by an accidental overgrinding of the apical area of the root, respectively. These artifacts were encountered occasionally even in ground sections prepared by highly trained personnel and became more frequent in ground sections prepared by a person who, though trained, did not use this technique daily. A modification of the data-collecting method is suggested to make possible the use of thick (1.0-mm) ground sections for the evaluation of most of the aging criteria, thereby eliminating the possible inaccuracies in the preparation and evaluation of the thin (0.25-mm) ground sections.
古斯塔夫森从牙齿判断年龄的方法基于对牙齿磨片的评估。在磨片中评估六个与年龄相关的参数，并与年龄与年龄相关变化的回归曲线进行比较。其中两个变化，即牙根牙本质和继发性牙本质的透明度，与年龄的相关性最高。仅从薄（0.25毫米）磨片评估这些参数可能会导致人为地得出较高的“继发性牙本质值”。这些假象可能分别是由于试图在0.25毫米厚的磨片中包含整个牙髓腔和根管，以及意外过度研磨牙根根尖区域造成的。即使在由训练有素的人员制备的磨片中，这些假象也偶尔会出现，而在由虽经训练但不每天使用该技术的人员制备的磨片中，这种情况更为频繁。建议对数据收集方法进行修改，以便能够使用厚（1.0毫米）磨片来评估大多数老化标准，从而消除薄（0.25毫米）磨片制备和评估中可能存在的不准确之处。
