曲霉TS对孕酮11α-羟基化作用的改进
An improved 11 alpha-hydroxylation of progesterone by Aspergillus ochraceus TS.
作者信息
Samanta T B, Roy N, Chattopadhyay S
出版信息
Biochem J. 1978 Nov 15;176(2):593-4. doi: 10.1042/bj1760593.
Abstract
- The stereospecific hydroxylation of progesterone exclusively to its 11 alpha-hydroxy derivative by Aspergillus ochraceus TS is reported. 2. There is no secondary metabolite (6beta, 11 alpha-dihydroxyprogesterone) formed even when the transformation was attempted with different concentrations of the substrate (200 microgram/ml-200 mg/ml) for prolonged with Zn2+ at a concentration of 50 microgram/ml identical results were obtained. 3. Similar results were also obtained at different pH values of the culture medium.
摘要
- 据报道，曲霉TS能将孕酮立体选择性地羟基化，专一生成其11α-羟基衍生物。2. 即使尝试用不同浓度的底物（200微克/毫升 - 200毫克/毫升）进行长时间转化，也不会形成次级代谢产物（6β, 11α-二羟基孕酮）；当添加浓度为50微克/毫升的Zn2+时，得到了相同的结果。3. 在培养基的不同pH值下也获得了类似的结果。
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本文引用的文献
1
Microbiological hydroxylation of steroids. X. 1 beta, 11 alpha-dihydroxylation of 3 beta-hydroxy-5 alpha-prepnan-20-one and the hydroxylation of other 20-oxo-5 alpha-pregnanes with the fungus Aspergillys ochraceus.甾体的微生物羟基化作用。X. 用曲霉对3β-羟基-5α-孕甾烷-20-酮进行1β, 11α-二羟基化以及对其他20-氧代-5α-孕甾烷的羟基化作用
J Chem Soc Perkin 1. 1973;19:2137-41.
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