Dutta T K, Samanta T B
Marine Biology Institute, 3-75-1 Heita, Kamaishi City, Iwate 026, Japan.
Curr Microbiol. 1999 Dec;39(6):309-0312. doi: 10.1007/s002849900464.
Progesterone was transformed to its 11alpha-hydroxy derivative (100% e.e) by the activated immobilized conidia of Aspergillus ochraceus TS. The immobilized preparation retained 79% of free conidial activity. The immobilized conidia, activated by nutrients, exhibited an increase in 11alpha-hydroxylation, and it was free of the side product 6beta, 11alpha-dihydroxy progesterone. The half life and turnover of immobilized and activated immobilized conidia were 14 and 12 days and 187 and 416 micromoles of the product/g of conidia respectively. The pH and temperature profiles of the free conidia remained unaltered after immobilization and activation. Some germination of conidia inside the matrix owing to incubation with nutrients was detected by scanning electron microscopy.
赭曲霉TS的固定化活化分生孢子将孕酮转化为其11α-羟基衍生物（对映体过量百分数为100%）。固定化制剂保留了游离分生孢子79%的活性。经营养物活化的固定化分生孢子表现出11α-羟基化作用增强，且没有副产物6β,11α-二羟基孕酮。固定化和活化固定化分生孢子的半衰期和周转量分别为14天和12天，以及每克分生孢子187微摩尔和416微摩尔产物。固定化和活化后，游离分生孢子的pH和温度曲线未发生改变。通过扫描电子显微镜检测到，由于与营养物一起孵育，基质内的一些分生孢子发生了萌发。
