Hamada H, Kominami R, Muramatsu M
Nucleic Acids Res. 1980 Feb 25;8(4):889-903.
The 3'-terminal structures of ribosomal 28S RNA and its precursors from rat and mouse were analyzed by means of periodate oxidation followed by reduction with 3H-borohydride. 3'-terminal labeled nucleoside derivatives produced by RNase T2 digestion were determined by thin-layer chromatography and oligonucleotides generated by RNase T1 digestion were analyzed by DEAE-Sephadex chromatography. In the rat, the major 3'-terminal sequences of ribosomal 28S RNA, nucleolar 28S, 32S, 41S, and 45S RNAs were YGUoh, GZ2Uoh, GZ12Uoh, GZ2Uoh, and GZ7Goh, respectively, whereas in the mouse corresponding sequences were YGUoh, GZ1,2, or 3Uoh, Goh, Uoh and GZ 13Uoh, respectively. (Y: pyrimidine nucleoside, Z: any nucleoside other than guanosine) These results suggest that a "transcribed spacer" sequence is present at the 3'-terminus of the 45S pre-ribosomal RNA, which is gradually removed during the steps of processing.
采用高碘酸盐氧化，随后用³H-硼氢化物还原的方法，分析了大鼠和小鼠核糖体28S RNA及其前体的3'末端结构。通过薄层层析法测定了核糖核酸酶T2消化产生的3'末端标记核苷衍生物，并通过二乙氨基乙基葡聚糖（DEAE-Sephadex）层析法分析了核糖核酸酶T1消化产生的寡核苷酸。在大鼠中，核糖体28S RNA、核仁28S、32S、41S和45S RNA的主要3'末端序列分别为YGUoh、GZ2Uoh、GZ12Uoh、GZ2Uoh和GZ7Goh，而在小鼠中，相应序列分别为YGUoh、GZ1、2或3Uoh、Goh、Uoh和GZ 13Uoh。（Y：嘧啶核苷，Z：除鸟苷以外的任何核苷）这些结果表明，在45S核糖体前体RNA的3'末端存在一个“转录间隔区”序列，该序列在加工过程中逐渐被去除。
